p-p53 Antibody (F-11): sc-377561
- p-p53 Antibody (F-11) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids Ser 46 Ser 46 of p53 of human origin
- p-p53 Antibody (F-11) is recommended for detection of Ser 46 phosporylated p53 of human origin by WB, IP, IF and ELISA
- Anti-p-p53 Antibody (F-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-p53 Antibody (F-11). This work is in progress.
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SEE ALSO...
p-p53 Antibody (F-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects p53, specifically the Ser 46 phosphorylated form, in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p-p53 monoclonal antibody (F-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. p53, a crucial tumor suppressor, plays a vital role in regulating cell cycle and promoting apoptosis in response to cellular stress, thereby acting as a guardian of genomic integrity. p53 is primarily located in the nucleus, where p53 binds to DNA and activates transcription of genes involved in growth arrest and apoptosis. Phosphorylation of p53 at Ser 46 enhances pro-apoptotic activity by selectively transactivating genes involved in programmed cell death, making this modification critical in cellular stress responses. This phosphorylation event predominantly occurs in the nucleus, where p53 orchestrates transcriptional programs, but can also influence subcellular localization and stability, further regulating tumor-suppressive functions. p53 can be sequestered in the cytoplasm by E3 ubiquitin ligase MDM2, which is a negative regulator that targets p53 for proteasomal degradation. Dynamic localization of p53 is essential for function, allowing rapid responses to cellular stress signals. p53 undergoes various post-translational modifications, including phosphorylation and acetylation, which are critical for activation and function. Mutations in the DNA-binding domain of p53 can lead to loss of function and are frequently associated with a wide range of human cancers, underscoring p53′s importance in tumor suppression and anti-p53 antibody (F-11)′s potential in cancer research and diagnostics.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-p53 Antibody (F-11) | sc-377561 | 200 µg/ml | $322.00 | |||
p-p53 Antibody (F-11) AC | sc-377561 AC | 500 µg/ml, 25% agarose | $424.00 | |||
p-p53 Antibody (F-11) HRP | sc-377561 HRP | 200 µg/ml | $322.00 | |||
p-p53 Antibody (F-11) FITC | sc-377561 FITC | 200 µg/ml | $336.00 | |||
p-p53 Antibody (F-11) PE | sc-377561 PE | 200 µg/ml | $349.00 | |||
p-p53 Antibody (F-11) Alexa Fluor® 488 | sc-377561 AF488 | 200 µg/ml | $364.00 | |||
p-p53 Antibody (F-11) Alexa Fluor® 546 | sc-377561 AF546 | 200 µg/ml | $364.00 | |||
p-p53 Antibody (F-11) Alexa Fluor® 594 | sc-377561 AF594 | 200 µg/ml | $364.00 | |||
p-p53 Antibody (F-11) Alexa Fluor® 647 | sc-377561 AF647 | 200 µg/ml | $364.00 | |||
p-p53 Antibody (F-11) Alexa Fluor® 680 | sc-377561 AF680 | 200 µg/ml | $364.00 | |||
p-p53 Antibody (F-11) Alexa Fluor® 790 | sc-377561 AF790 | 200 µg/ml | $364.00 | |||
p-p53 (F-11) Neutralizing Peptide | sc-377561 P | 100 µg/0.5 ml | $69.00 |