p-eNOS Antibody (pT495.33): sc-136519

p-NOS3 Antibody (pT495.33) is a mouse monoclonal IgG2a kappa light chain antibody that detects the Thr 495 phosphorylated NOS3 protein of human origin, as well as the corresponding Thr 492 phosphorylated NOS3 in mouse and rat, demonstrating reactivity across mouse, rat, and human species by western blotting (WB) and immunoprecipitation (IP). anti-p-NOS3 antibody (pT495.33) is available in both conjugated and non-conjugated forms, providing flexibility for various experimental needs. The endothelial nitric oxide synthase (NOS3) plays a crucial role in cardiovascular health by producing nitric oxide (NO), a signaling molecule essential for maintaining vascular tone and blood pressure. NOS3 regulates processes such as vascular remodeling and angiogenesis, which are critical for tissue repair and response to injury. NOS3 activation is intricately linked to phosphorylation status, particularly at serine 1177, which is mediated by the serine/threonine protein kinase Akt/PKB, a key player in signaling pathways activated by insulin-like growth factor-1 and vascular endothelial growth factor (VEGF). Phosphorylation at threonine 495, influenced by protein kinase C (PKC), can modulate NOS3 activity, highlighting complex regulatory mechanisms governing function. Understanding these phosphorylation events is essential for elucidating NOS3′s role in cardiovascular diseases and developing targeted therapies.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-eNOS Antibody (pT495.33) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-p-eNOS Antibody (pT495.33) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-eNOS Antibody (pT495.33) Specifications

Alternate Product Name: NOS3

Product Clone Name: pT495.33

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: Thr 495

Epitope Target: NOS3

Epitope Species: human

Product Reactivity/Detection: Thr 495 phosphorylated NOS3 of human origin and correspondingly Thr 492 phosphorylated NOS3 of mouse and rat origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for p-eNOS Antibody (pT495.33): AC: Agarose, HRP: Horseradish Peroxidase, X: TransCruz Reagent

The preferred eNOS antibody is eNOS Antibody (A-9): sc-376751.