p-GATA4 Antibody (H-4): sc-377543

p-GATA-4 Antibody (H-4) is a mouse monoclonal IgM antibody that detects Ser 262 phosphorylated GATA-4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-p-GATA-4 antibody (H-4) is available in a non-conjugated form, allowing for versatile experimental use. GATA-4, a member of the GATA transcription factor family, plays a crucial role in cardiac development and function, as GATA-4 is essential for regulating genes involved in heart morphogenesis and differentiation. GATA-4 is primarily located in the nucleus, where GATA-4 binds to specific DNA sequences to activate or repress target gene expression, thereby influencing cellular processes such as proliferation and apoptosis. GATA-4′s nuclear localization ensures transcriptional regulation occurs precisely where needed, allowing for proper development of cardiac tissues and maintenance of heart function. GATA-4 interacts with other proteins, including GATA-6 and various co-factors, which further modulates GATA-4′s activity and expands GATA-4′s regulatory network. Understanding GATA-4 dynamics and interactions is vital for elucidating mechanisms underlying cardiac diseases and potential therapeutic targets.

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p-GATA4 Antibody (H-4) References:

1. Hepatocyte growth factor induces GATA-4 phosphorylation and cell survival in cardiac muscle cells.  |  Kitta, K., et al. 2003. J Biol Chem. 278: 4705-12. PMID: 12468531
2. Human transcription factor GATA-2. Evidence for regulation of preproendothelin-1 gene expression in endothelial cells.  |  Dorfman, DM., et al. 1992. J Biol Chem. 267: 1279-85. PMID: 1370462
3. Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer.  |  Ko, LJ., et al. 1991. Mol Cell Biol. 11: 2778-84. PMID: 2017177
4. Molecular role of GATA binding protein 4 (GATA-4) in hyperglycemia-induced reduction of cardiac contractility.  |  Ku, PM., et al. 2011. Cardiovasc Diabetol. 10: 57. PMID: 21702924
5. Increase in cardiac M2-muscarinic receptor expression is regulated by GATA binding protein 4 (GATA-4) in streptozotocin-induced diabetic rats.  |  Mar, GY., et al. 2013. Int J Cardiol. 167: 436-41. PMID: 22293779
6. Erythropoietin-activated ERK/MAP kinase enhances GATA-4 acetylation via phosphorylation of serine 261 of GATA-4.  |  Jun, JH., et al. 2013. J Cell Physiol. 228: 190-7. PMID: 22674427
7. Erythropoietin prevents hypoxia-induced GATA-4 ubiquitination via phosphorylation of serine 105 of GATA-4.  |  Jun, JH., et al. 2013. Biol Pharm Bull. 36: 1126-33. PMID: 23811561
8. GATA-4/5/6, a subfamily of three transcription factors transcribed in developing heart and gut.  |  Laverriere, AC., et al. 1994. J Biol Chem. 269: 23177-84. PMID: 8083222
9. DNA-binding specificities of the GATA transcription factor family.  |  Ko, LJ. and Engel, JD. 1993. Mol Cell Biol. 13: 4011-22. PMID: 8321208
10. The human GATA-6 gene: structure, chromosomal location, and regulation of expression by tissue-specific and mitogen-responsive signals.  |  Suzuki, E., et al. 1996. Genomics. 38: 283-90. PMID: 8975704

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-GATA4 Antibody (H-4) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-p-GATA4 Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-GATA4 Antibody (H-4) Specifications

Alternate Product Name: GATA4

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: Ser 262

Epitope Target: GATA-4

Epitope Species: human

Product Reactivity/Detection: Ser 262 phosphorylated GATA-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for p-GATA4 Antibody (H-4): P: Blocking Peptide

The preferred GATA4 antibody is GATA4 Antibody (G-4): sc-25310.