p-Akt1/2/3 Anticuerpo (C-11) recomendado para detectarSer 473 phosphorylated Akt1 and correspondingly Ser 474 phosphorylated Akt2 and correspondingly Ser 472 phosporylated Akt3 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA

p-Akt1/2/3 Anticuerpo (C-11): sc-514032

p-Akt1/2/3 Anticuerpo (C-11) clasificado 4.5 de 5 por 55.
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Ficha técnica

    • p-Akt1/2/3 Anticuerpo (C-11) es un monoclonal de ratón IgG2b (kappa light chain) proporcionado como 200 µg/ml
    • specific for an epitope mapping to a short amino acid sequence containing phosphorylated Ser 473 of Akt1 of human origin
    • p-Akt1/2/3 Anticuerpo (C-11) es recomendado para detectar Ser 473 phosphorylated Akt1 and correspondingly Ser 474 phosphorylated Akt2 and correspondingly Ser 472 phosporylated Akt3 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA
    • p-Akt1/2/3 Anticuerpo (C-11) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • péptido de bloqueo, sc-514032 P
    • Ver m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nuestras altamente recomendadas alternativa a los anticuerpos secundarios convencionales, anti-IgG de ratón.
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de p-Akt1/2/3 (C-11): sc-514032.
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HRP 의 경우 타겟 항체에 대한 specific 제품을 사용해야 하나요 ?  p-Akt 항체를 가지고 WB를 하려고 하는데, p-Akt1/2/3 Antibody (C-11) HRP 또는 m-IgGκ BP-HRP sc-516102을 사용해도 되는지요 ? 

Preguntado por: seong Joseph
산타크루즈바이오테크놀러지에 문의 주셔서 감사합니다.HRP 결합 1 차 항체 있으시면 ,2 차 항체 사용할 필요가 없습니다.sc-514032는 sc-516102을 사용해도 됍니다.다른 문제 있으시면 기술지원팀에 연락하셔서 전화:86 21-60936350 이메일: [email protected] 또는 온라인문의로 문의하시면 됩니다. Thank you for your question.There is no need to use 2nd antibody with HRP conjugated primary antibody.sc-51610 is a suitble 2nd antibody for sc-514032 in WB.If you have any other question ,please contact our Technical Service Department at email [email protected],call us directly at  (86 21) 6093-6350, or contact us by live chat.
Respondida por: Technical Support 8
Fecha de publicación: 2019-01-25

I am using p-Akt1/2/3 (C-11): sc-514032 monoclonal antibody for Western blot. How do I know if the band I am detecting is specific?

Preguntado por: jenni
We recommend using the blocking peptide sc-514032 P as a negative control. Antibody binding to antigen may be blocked/competed by pre-absorption with the blocking peptide. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Respondida por: Technical Support 10
Fecha de publicación: 2017-01-12

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with p-Akt1/2/3 (C-11): sc-514032?

Preguntado por: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Respondida por: Technical Support 10
Fecha de publicación: 2017-01-12
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Rated 4 de 5 por de Very good antibody The antibody was used for Western Blot with breast carcinoma cells, it works very well at a concentration of 1: 200.
Fecha de publicación: 2018-11-22
Rated 5 de 5 por de It works great for western blot. sc-514032 works great for western blot with HUVECs cell lines.
Fecha de publicación: 2018-11-12
Rated 5 de 5 por de Very good Good on IHC staining of tumor epithelial cells, 1:100 dilution.
Fecha de publicación: 2018-07-24
Rated 5 de 5 por de great bands it's works great on my western blot! clear bands at 58kDa
Fecha de publicación: 2018-04-03
Rated 2 de 5 por de Couldn't get it to work for WB Maybe this could work with more optimization. But I ran out of sample so could not optimize. I was using 1:200 dilution in mouse samples.
Fecha de publicación: 2018-03-22
Rated 5 de 5 por de Funziona molto bene Ho ordinato il capione di prova e subito l'ho provato. Ho diluito l'anticorpo 1:500 in PBS+BSA. Il segnale che ho ottenuto è stato forte e pulito, solo un aspecifico sopra i 90 KDa. Un buon anti mouse, consigliato!
Fecha de publicación: 2018-01-19
Rated 4 de 5 por de gute Ergebniss in WB. gute Ergebnis in WB. I need to adjust the result a Little to get clear band, but this product do work well for WB.
Fecha de publicación: 2018-01-15
Rated 4 de 5 por de guter Antikörper für Western Blot Antikörper 1:500 verdünnt, gute Banden mit etwas Hintergrund Zellylsate
Fecha de publicación: 2017-11-17
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