NUAK1/ARK5 CRISPR/Cas9 KO Plasmid (m): sc-429607
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NUAK1/ARK5 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NUAK1/ARK5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NUAK1/ARK5 CRISPR/Cas9 KO Plasmid (m) | sc-429607 | 20 µg | $397.00 |
Overview
Nuak1 encodes NUAK1/ARK5, a serine/threonine kinase in the AMPK-related kinase family that links cellular energy status to adaptive programs controlling survival, polarity, adhesion, and cytoskeletal remodeling. NUAK1 signaling has been associated with regulation of myosin phosphatase activity, stress-responsive kinase cascades, and coordination of metabolic responses under nutrient limitation. In mouse systems, NUAK1/ARK5 activity is used to study how energy-sensing pathways interface with migration, invasion-like behaviors, and resistance to stress-induced apoptosis. Dysregulated NUAK1/ARK5 function has been implicated in oncogenic and metabolic phenotypes, supporting its use as a mechanistic node in models of tumor biology and cellular stress.
NUAK1/ARK5 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nuak1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nuak1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nuak1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NUAK1/ARK5 protein expression.
This CRISPR knockout system enables efficient generation of Nuak1-deficient cell models for investigation of NUAK1/ARK5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nuak1 exon(s) critical for NUAK1/ARK5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nuak1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NUAK1/ARK5 CRISPR/Cas9 KO Plasmid (m) and NUAK1/ARK5 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nuak1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NUAK1/ARK5 HDR Plasmid (m) and NUAK1/ARK5 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nuak1 homology arms to support homology-directed repair at defined Nuak1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.