Nkx-6.1 CRISPR/Cas9 KO Plasmid (h): sc-401997
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nkx-6.1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Nkx-6.1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nkx-6.1 Antibody (5B8): sc-130385
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nkx-6.1 CRISPR/Cas9 KO Plasmid (h) | sc-401997 | 20 µg | $397.00 |
Overview
NKX6-1 encodes the homeobox transcription factor Nkx-6.1, a key regulator of pancreatic endocrine lineage specification and β-cell maturation. Nkx-6.1 coordinates transcriptional programs controlling insulin secretion, glucose-stimulated metabolic coupling, and maintenance of β-cell identity through networks that intersect with PDX1-, NEUROD1-, and MAFA-associated gene regulation. Altered NKX6-1 expression or activity has been linked to impaired β-cell function and dysregulated glucose homeostasis, making it relevant for studies of diabetes-related mechanisms and endocrine development. Beyond pancreas biology, Nkx-6.1 is used as a marker and functional node in differentiation models that probe lineage commitment and transcriptional control.
Nkx-6.1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NKX6-1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NKX6-1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NKX6-1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Nkx-6.1 protein expression.
This CRISPR knockout system enables efficient generation of NKX6-1-deficient cell models for investigation of Nkx-6.1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NKX6-1 exon(s) critical for Nkx-6.1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NKX6-1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Nkx-6.1 CRISPR/Cas9 KO Plasmid (h) and Nkx-6.1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NKX6-1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Nkx-6.1 HDR Plasmid (h) and Nkx-6.1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NKX6-1 homology arms to support homology-directed repair at defined NKX6-1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.