NIPSNAP1/2 Antibody (F-4): sc-393201

NIPSNAP1/2 Antibody (F-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects NIPSNAP1/2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). NIPSNAP1/2 (F-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NIPSNAP1 protein, which is 228 amino acids long, plays a crucial role in modulating the activity of the transient receptor potential vanilloid 6 (TRPV6) channel, a key player in calcium homeostasis. By inhibiting TRPV6 currents, NIPSNAP1 regulates calcium entry across the plasma membrane in tissues such as the pancreas and placenta, which is vital for numerous physiological processes, including muscle contraction and neurotransmitter release. NIPSNAP1 does not alter the membrane expression of TRPV6, indicating that NIPSNAP1′s inhibitory effect is mediated through a mechanism that does not involve reducing the number of channels at the cell surface. NIPSNAP2, also known as glioblastoma amplified sequence (GBAS), is a 286 amino acid protein that is predominantly expressed in heart and skeletal muscle, suggesting NIPSNAP2′s involvement in vesicular transport and cellular signaling pathways. With diverse applications and critical role in calcium regulation, anti-NIPSNAP1/2 antibody (F-4) serves as an essential tool for researchers studying these important proteins.

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NIPSNAP1/2 Antibody (F-4) References:

1. Identification of a NIPSNAP homologue as host cell target for Salmonella virulence protein SpiC.  |  Lee, AH., et al. 2002. Cell Microbiol. 4: 739-50. PMID: 12427096
2. Expression pattern and raft association of NIPSNAP3 and NIPSNAP4, highly homologous proteins encoded by genes in close proximity to the ATP-binding cassette transporter A1.  |  Buechler, C., et al. 2004. Genomics. 83: 1116-24. PMID: 15177564
3. Expression of calpastatin, minopontin, NIPSNAP1, rabaptin-5 and neuronatin in the phenylketonuria (PKU) mouse brain: possible role on cognitive defect seen in PKU.  |  Surendran, S., et al. 2005. Neurochem Int. 46: 595-9. PMID: 15863237
4. Identification of Nipsnap1 as a novel auxiliary protein inhibiting TRPV6 activity.  |  Schoeber, JP., et al. 2008. Pflugers Arch. 457: 91-101. PMID: 18392847
5. [Identification of proteins in complexes with alpha-latrotoxin receptors].  |  Serova, OV., et al. 2008. Bioorg Khim. 34: 747-53. PMID: 19088747
6. NIPSNAP1 and NIPSNAP2 Act as 'Eat Me' Signals for Mitophagy.  |  Princely Abudu, Y., et al. 2019. Dev Cell. 49: 509-525.e12. PMID: 30982665
7. NIPSNAP1 and NIPSNAP2 act as 'eat me' signals to allow sustained recruitment of autophagy receptors during mitophagy.  |  Abudu, YP., et al. 2019. Autophagy. 15: 1845-1847. PMID: 31251109
8. The Tumorigenic Effect of lncRNA AFAP1-AS1 is Mediated by Translated Peptide ATMLP Under the Control of m6 A Methylation.  |  Pei, H., et al. 2023. Adv Sci (Weinh). 10: e2300314. PMID: 36871154
9. NIPSNAP1 directs dual mechanisms to restrain senescence in cancer cells.  |  Gao, E., et al. 2023. J Transl Med. 21: 401. PMID: 37340421
10. SIRT3 regulates mitophagy in liver fibrosis through deacetylation of PINK1/NIPSNAP1.  |  Li, R., et al. 2023. J Cell Physiol. 238: 2090-2102. PMID: 37417912
11. Nipsnap1-A regulatory factor required for long-term maintenance of non-shivering thermogenesis.  |  Liu, Y., et al. 2023. Mol Metab. 75: 101770. PMID: 37423391
12. Characterization of the human NIPSNAP1 gene from 22q12: a member of a novel gene family.  |  Seroussi, E., et al. 1998. Gene. 212: 13-20. PMID: 9661659

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NIPSNAP1/2 Antibody (F-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-NIPSNAP1/2 Antibody (F-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NIPSNAP1/2 Antibody (F-4) Specifications

Alternate Product Name: NIPSNAP1/2

Product Clone Name: F-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 197-284

Epitope Target: NIPSNAP1

Epitope Species: mouse

Product Reactivity/Detection: NIPSNAP1 and NIPSNAP2 of mouse and human origin, and the corresponding rat homologs

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for NIPSNAP1/2 Antibody (F-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred NIPSNAP1 antibody is NIPSNAP1 Antibody (H-9): sc-515197.