NHERF-1 Double Nickase Plasmid (m): sc-424152-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NHERF-1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NHERF-1 Double Nickase Plasmid (m) and NHERF-1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Slc9a3r1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NHERF-1 Antibody (A-7): sc-271552
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NHERF-1 Double Nickase Plasmid (m) | sc-424152-NIC | 20 µg | $410.00 |
Slc9a3r1 encodes NHERF-1, a PDZ domain–containing scaffolding protein that organizes membrane signaling complexes and couples transporters and receptors to the actin cytoskeleton. In mouse cells, NHERF-1 coordinates processes such as ion transport and receptor trafficking by interacting with proteins including Na⁺/H⁺ exchangers, GPCRs, and receptor tyrosine kinases, thereby influencing downstream pathways that control epithelial polarity and signal integration. Through these scaffold functions, NHERF-1 helps regulate phosphoinositide signaling dynamics and cytoskeletal remodeling at the plasma membrane. Dysregulation of NHERF-1–dependent complexes has been linked in the literature to altered epithelial homeostasis and signaling programs relevant to renal and gastrointestinal physiology and to oncogenic pathway modulation in model systems.
NHERF-1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Slc9a3r1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Slc9a3r1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Slc9a3r1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Slc9a3r1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.