Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NGFR p75 Double Nickase Plasmid (h): sc-400552-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NGFR p75 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NGFR p75 Double Nickase Plasmid (h) and NGFR p75 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NGFR. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NGFR p75 Antibody (B-1): sc-271708
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NGFR p75 Double Nickase Plasmid (h) | sc-400552-NIC | 20 µg | $410.00 | |||
NGFR p75 Double Nickase Plasmid (h2) | sc-400552-NIC-2 | 20 µg | $410.00 |
NGFR encodes the p75 neurotrophin receptor (NGFR p75), a member of the TNF receptor superfamily that binds neurotrophins and proneurotrophins to modulate neuronal survival, apoptosis, neurite outgrowth, and synaptic remodeling. NGFR p75 functions as a context-dependent signaling hub through interactions with coreceptors such as Trk receptors and sortilin, engaging pathways including NF-κB, JNK/c-Jun, RhoA-mediated cytoskeletal dynamics, and ceramide signaling. Beyond the nervous system, NGFR p75 influences Schwann cell biology, axon regeneration, and neural crest–derived cell states. Dysregulated NGFR signaling and expression have been associated with neurodegenerative processes, nerve injury responses, and tumor cell plasticity and invasiveness in several cancer-relevant contexts, supporting its use in mechanistic studies.
NGFR p75 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NGFR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NGFR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NGFR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NGFR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.