Date published: 2025-9-8

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Neurofibromin Anticuerpo (McNFn27a): sc-20017

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Fichas Técnicas
  • Neurofibromin Anticuerpo (McNFn27a) es un monoclonal de ratón IgG1 κ, ver las 4 publicaciones, proporcionado como 200 µg/ml
  • criados contra un péptido N-terminal de Neurofibromin de human origen
  • recomendado para detectarNeurofibromin de mouse, rat y human origen, mediante WB, IP, IF y IHC(P)
  • Ver Neurofibromin (H-12): sc-376886 para Neurofibromin anticuerpos conjugados, incluyendo AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 y 790.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP y m-IgGκ BP-HRP son los reactivos de detección secundarios preferidos para Neurofibromin Anticuerpo (McNFn27a) para aplicactiones WB and IHC(P). Estos reactivos se ofrecen ahora en paquetes con Neurofibromin Anticuerpo (McNFn27a) (ver información de pedido más abajo).

ENLACES RÁPIDOS

VER TAMBIÉN ....

La neurofibromatosis tipo 1 (NF1), o neurofibromatosis de von Recklinghausen, es uno de los trastornos autosómicos dominantes más comunes en los seres humanos. El análisis de ligamiento temprano mapeó el gen NF1 al cromosoma 17. El transcripto predicho de NF1 codifica la proteína Neurofibromina de 2,818 aminoácidos, también designada como proteína relacionada con NF1-GAP (NF1GRP). El análisis de secuencia ha demostrado similitud dentro de una pequeña región de Neurofibromina y miembros de la familia de genes Ras GAP. Funcionalmente, se ha demostrado que Neurofibromina se asemeja a una proteína GAP mediante análisis bioquímicos que involucran la hidrólisis de RAS-GAP y la complementación funcional en levaduras. La proteína Neurofibromina se expresa a niveles relativamente constantes en una amplia gama de líneas celulares y tejidos, incluyendo cerebro, pulmón, hígado, riñón, bazo, músculo y colon. Aunque se sabe poco sobre la función de Neurofibromina, la homología con el dominio catalítico de proteínas con actividad de GTPasa sugiere que Neurofibromina también puede interactuar in vivo con proteínas Ras.

Para uso exclusivo en Investigación No está diseñado para para Diagnóstico ó Terapia.

Información sobre pedidos

Nombre del productoNúmero de catálogoUNIDADPrecioCANTIDADFavoritos

Neurofibromin Anticuerpo (McNFn27a)

sc-20017
200 µg/ml
$316.00

Paquete de Neurofibromin (McNFn27a): m-IgG Fc BP-HRP

sc-536640
200 µg Ab; 10 µg BP
$354.00

Paquete de Neurofibromin (McNFn27a): m-IgGκ BP-HRP

sc-533797
200 µg Ab; 40 µg BP
$354.00

Paquete de Neurofibromin (McNFn27a): m-IgG1 BP-HRP

sc-544725
200 µg Ab; 20 µg BP
$354.00

I am using Neurofibromin (McNFn27a): sc-20017 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Preguntado por: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Respondida por: Technical Support
Fecha de publicación: 2017-02-24
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Rated 5 de 5 por de This antibody genuinely *does* work (even on WB)Hi. I am first author of one of the cited publications for this antibody (at least my pub still listed as 08/2025; Kahen, E. et al.) and I noticed someone else said they could not get signal on WB in their hands. I want to reassure that you really can get great WB's with this antibody for any human cell line, but it is a labor of love. Neurofibromin is a fairly large protein as you likely know, (typically considered 240 kd) often running somewhere around 210-240 kda range on a gel per MW markers. As I wrote in my methods, I had to use a tris-acetate gradient gel of 3-8% to capture NF1 and its putative isoform explicitly (Very painful gel to deal with; many gels were broken during troubleshooting). Getting the loading control was tricky because tubulin would run around the same range as a non-specific band. Alpha-actinin ran *very close* to a non-specific band so was not "publication worthy" but was good for testing, and was used, and others might benefit from this using alpha-actinin for loading control for NF1 if new antibodies more specific. I had to find perfect timing to be able to capture proper *actin* right at the bottom of the gel, which has far more specific WB antibodies for pub-worthy images. Note: Wet transfer 100% necessary to get all the NF1. Dry transfer would either over-transfer the actin or (most often) under-transfer the NF1. Very difficult to get sufficient NF1 on a membrane via dry-transfer without losing other bands for controls (and I was also really trying to get RAS on same gel). Honestly I think I legit tried dry transfer for a hilarious amount of time once and when I stained the gel out of curiosity, there was *still* so much protein in the NF1 region. I really tried many times with dry transfer- wet transfer only way to get anything useful. Then, I actually blocked membranes *overnight* in Odyssey blocking buffer. This Santa-Cruz NF1 antibody was then used at highest concentration recommended by manufacturer, again in Odyssey blocking buffer *overnight* (4degC on shaker). Very thorough washing in TBST (and I think I actually preferred lower tween 20 % but 10 min washes). I really wanted clean crisp neurofibromin showing two bands, as I did. And ultimately, I was able to get the result I wanted and reproduce it (though actin could really have looked a little prettier, but quantified accurately overall.) I suppose we should really be this detailed in our methods, explaining these sorts of things, but we tend not to be. Nonetheless, what I've described might help someone working with this antibody. Feel free to reach out via email and I will try to remember my best other than what I've written here.
Fecha de publicación: 2025-08-02
Rated 1 de 5 por de Not working for WB in our handsessentially no specific bands, nor non-specific bands.
Fecha de publicación: 2019-05-29
Rated 4 de 5 por de WB: Worked well in mouse spleen samplesWB: Worked well in mouse spleen samples. -SCBT Publication Review
Fecha de publicación: 2015-06-18
Rated 5 de 5 por de Published data for IF in human Schwann cellsPublished data for IF in human Schwann cells and CCF cells. -SCBT Publication Review
Fecha de publicación: 2013-05-05
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Neurofibromin Anticuerpo (McNFn27a) clasificado 3.8 de 5 por 4.
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