Nanog Anticuerpo (5A10) recomendado para detectarNanog de mouse, rat y human origen, mediante WB, IP y ELISA

Nanog Anticuerpo (5A10): sc-134218

Nanog Anticuerpo (5A10) clasificado 4.9 de 5 por 7.
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Ficha técnica

    • Nanog Anticuerpo 5A10 es un monoclonal de ratón IgG2a, ver las 23 publicaciones, proporcionado como 50 µg/500 µl
    • raised against amino acids 1-154 corresponding to recombinant Nanog of human origin
    • recomendado para detectarNanog de mouse, rat y human origen, mediante WB, IP y ELISA
    • Ver Nanog (1E6C4): sc-293121 para Nanog anticuerpos conjugados, incluyendo AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 y 790.
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de Nanog (5A10): sc-134218.

Is this Nanog (5A10): sc-134218 monoclonal antibody available directly conjugated to HRP?

Preguntado por: cjMara
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected] Alternatively, consider using Nanog (1E6C4): sc-293121, which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/nanog-antibody-1e6c4)
Respondida por: Technical Support
Fecha de publicación: 2017-01-30
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Rated 4 de 5 por de Buon funzionamento Ho provato l' anticorpo primario Nanog (5A10):sc-134218. Lo ho usato per western blot e devo dire che il risultato è stato soddisfacente. Ho usato una concentrazione (1:2000) più bassa di quella suggerita (1:1000) ed ho avuto un buon risultato lo stesso su due membrane su cui avevo corso 30µ e 40 µg di proteine. L’incubazione con il primario è stata fatta ON a 4°C ed ho usato un secondario anti mouse 1:20000. Le bande sono nette e c’è poco aspecifico. Dopo l’incubazione con primario e dopo l’incubazione con secondario ho eseguito 3x10 min di lavaggi con TPBS (PBS+0.1% Tween) ed il blocking è stato fatto per 4 ore con Milk al 5% in TPBS a temperatura ambiente. Ritengo sia un prodotto valido per il mio tipo di ricerca e per il western blot in generale.
Fecha de publicación: 2020-07-21
Rated 5 de 5 por de very pleased That was a very good antibody, diluted 1:1000 in human ovary tissue , we obtained good results.
Fecha de publicación: 2017-05-11
Rated 5 de 5 por de great Clean bands obtained in western blot using human ovary tissue extract.
Fecha de publicación: 2017-04-17
Rated 5 de 5 por de Produced publishable WB data on human and mouse Well characterized with publications in the primary literature for WB in human and mouse samples
Fecha de publicación: 2017-01-11
Rated 5 de 5 por de WB: strong WB: strong, clean band in NIH/3T3 whole cell lysate. -SCBT QC
Fecha de publicación: 2015-03-22
Rated 5 de 5 por de Positive results in WB with transfected lysates Positive results in WB with transfected lysates. -SCBT QC
Fecha de publicación: 2015-02-03
Rated 5 de 5 por de Clean bands in western blot analysis of Nanog Clean bands in western blot analysis of Nanog expression in HeLa and Raji whole cell lysates and human ovary tissue extract. -SCBT QC
Fecha de publicación: 2013-08-25
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