N4BP1 Double Nickase Plasmid (h): sc-406515-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- N4BP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- N4BP1 Double Nickase Plasmid (h) and N4BP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting N4BP1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
N4BP1 Double Nickase Plasmid (h) | sc-406515-NIC | 20 µg | $410.00 | |||
N4BP1 Double Nickase Plasmid (h2) | sc-406515-NIC-2 | 20 µg | $410.00 |
N4BP1 (NEDD4 binding protein 1) is a cytosolic regulatory factor implicated in ubiquitin-dependent signaling through its interaction with NEDD4-family E3 ligases. It has been linked to control of inflammatory and innate immune pathways, including modulation of NF-κB–associated responses and cellular activation states in immune cells. By influencing protein turnover and signaling complex assembly, N4BP1 can affect transcriptional programs, stress responses, and context-dependent cell survival. Dysregulated N4BP1-associated signaling has been explored in studies of immune dysregulation and inflammation-related disease mechanisms.
N4BP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the N4BP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within N4BP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt N4BP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of N4BP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.