MK CRISPR/Cas9 KO Plasmid (m): sc-421609
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MK CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MK genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MK CRISPR/Cas9 KO Plasmid (m) | sc-421609 | 20 µg | $397.00 |
Overview
Mdk encodes midkine (MK), a secreted heparin-binding growth factor that regulates cell proliferation, survival, migration, and neurite outgrowth during development and tissue remodeling. MK signals through cell-surface proteoglycans and receptor complexes to modulate pathways linked to MAPK/ERK, PI3K/AKT, and inflammatory chemokine networks, influencing angiogenesis and extracellular matrix interactions. In mouse models, altered Mdk expression is associated with dysregulated immune cell recruitment and aberrant repair responses, providing relevance to studies of inflammation, fibrosis, and tumor-associated stromal signaling. MK also contributes to nervous system patterning and injury responses, making it a useful node for investigating neuroinflammatory and regenerative processes.
MK CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Mdk gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Mdk together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Mdk open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MK protein expression.
This CRISPR knockout system enables efficient generation of Mdk-deficient cell models for investigation of MK signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Mdk exon(s) critical for MK function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Mdk genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MK CRISPR/Cas9 KO Plasmid (m) and MK CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Mdk locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MK HDR Plasmid (m) and MK HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Mdk homology arms to support homology-directed repair at defined Mdk target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.