MIF Anticuerpo (D-2) recomendado para detectarMIF de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA

MIF Anticuerpo (D-2): sc-271631

MIF Anticuerpo (D-2) clasificado 4.4 de 5 por 11.
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Ficha técnica

    • Anti-MIF Anticuerpo D-2 es un monoclonal de ratón IgM κ MIF Anticuerpo, ver las 10 publicaciones, proporcionado como 200 µg/ml
    • específico para un epítopo localizado entre los amino ácidos 7-39 en el N-terminus de MIF de origen human
    • recomendado para detectarMIF de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA; también reactivo con otras especies, incluyendo porcine
    • disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; ficoeritrina, fluoresceina, Alexa Fluor® 488 ó Alexa Fluor® 647 para IF, IHC(P) y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de MIF (D-2): sc-271631.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MIF Antibody (D-2). This work is in progress.
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Is this antibody suitable for neutralization experiments in mice?

Preguntado por: Al2995
Thank you for your question. We have not tested the antibody for neutralization experiments and, therefore, we are uncertain if it will work in that application.
Respondida por: Santa Cruz TS
Fecha de publicación: 2021-07-10

What application is the blocking peptide sc-271631 P appropriate for?

Preguntado por: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Respondida por: Technical Support
Fecha de publicación: 2017-02-24
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Rated 4 de 5 por de Good antibody! Works well enough! I got strong bands at 1:500 dilution using 40ug of protein
Fecha de publicación: 2018-11-08
Rated 5 de 5 por de Excellent For WB In MCF7 and T47D cells, diluted at 1:1000, using tricine SDS PAGE, a very specific band was abserved. Excellent antibody.
Fecha de publicación: 2018-04-12
Rated 1 de 5 por de DId not detect mouse MIF (HT22 cells) Unfortunately, the antibody did not detect mouse MIF (HT22 & MEF cells) or rat MIF (primary rat cortex tissue) but human MIF (HEK293T cells).
Fecha de publicación: 2018-01-08
Rated 5 de 5 por de WB detection We usually used this antibody for WB detection MIF protein,we got the specific stainingat dilution 1:250,it's shows good quality.
Fecha de publicación: 2017-09-03
Rated 4 de 5 por de Work in IHC Good staining with low background using IHC in mouse tissue
Fecha de publicación: 2017-08-28
Rated 5 de 5 por de Good for WB 1:500 diluted for wb of rat protein which extracted from h9c2,the band is strong ,but there exist another band below the main band.
Fecha de publicación: 2017-08-08
Rated 5 de 5 por de Strong and reliable for IHC and WB Excellent choice if you need a dependable MIF antibody for immunohistochemistry or Western blot. Smart choice!
Fecha de publicación: 2017-02-06
Rated 4 de 5 por de Decent band in ARPE-19 cell lysates Decent band in ARPE-19 cell lysates, correct molecular weight. Couple higher molecular weight non-specific bands.
Fecha de publicación: 2016-10-05
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