MBOAT1 CRISPR/Cas9 KO Plasmid (h): sc-406397
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MBOAT1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MBOAT1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MBOAT1 CRISPR/Cas9 KO Plasmid (h) | sc-406397 | 20 µg | $397.00 |
Overview
MBOAT1 (membrane bound O-acyltransferase domain containing 1) encodes an endoplasmic reticulum–associated acyltransferase implicated in lipid remodeling through transfer of fatty acyl groups to hydroxylated substrates, influencing membrane lipid composition and cellular lipid homeostasis. By modulating the balance of bioactive lipids and membrane properties, MBOAT1 can affect signaling processes linked to metabolic regulation, organelle function, and cellular stress responses. Altered activity of membrane-bound acyltransferases has been explored in contexts such as dyslipidemia, inflammatory signaling, and tumor biology, where shifts in lipid composition can reshape proliferative and survival pathways. As a result, MBOAT1 is a useful target for dissecting lipid-driven mechanisms that couple membrane remodeling to downstream cellular phenotypes.
MBOAT1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MBOAT1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MBOAT1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MBOAT1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MBOAT1 protein expression.
This CRISPR knockout system enables efficient generation of MBOAT1-deficient cell models for investigation of MBOAT1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MBOAT1 exon(s) critical for MBOAT1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MBOAT1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MBOAT1 CRISPR/Cas9 KO Plasmid (h) and MBOAT1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MBOAT1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MBOAT1 HDR Plasmid (h) and MBOAT1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MBOAT1 homology arms to support homology-directed repair at defined MBOAT1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.