LEREPO4 CRISPR/Cas9 KO Plasmid (m): sc-427290
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LEREPO4 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LEREPO4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LEREPO4 CRISPR/Cas9 KO Plasmid (m) | sc-427290 | 20 µg | $397.00 |
Overview
Zc3h15 encodes the mouse protein LEREPO4, a predicted zinc-finger RNA-binding factor implicated in post-transcriptional gene regulation, including control of mRNA stability, processing, and translation. By shaping RNA fate, Zc3h15 is positioned to influence signaling-dependent transcriptional outputs and cellular programs such as proliferation, differentiation, and stress responses. Dysregulation of RNA-binding proteins and associated ribonucleoprotein complexes is broadly linked to altered immune signaling and oncogenic phenotypes, making Zc3h15 a relevant node for mechanistic studies of gene expression control. In mouse model systems, perturbation of Zc3h15 can be used to interrogate how RNA-centric regulation modulates pathway activity and cell-state transitions.
LEREPO4 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Zc3h15 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Zc3h15 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Zc3h15 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LEREPO4 protein expression.
This CRISPR knockout system enables efficient generation of Zc3h15-deficient cell models for investigation of LEREPO4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Zc3h15 exon(s) critical for LEREPO4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Zc3h15 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LEREPO4 CRISPR/Cas9 KO Plasmid (m) and LEREPO4 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Zc3h15 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LEREPO4 HDR Plasmid (m) and LEREPO4 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Zc3h15 homology arms to support homology-directed repair at defined Zc3h15 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.