LEF-1 Anticuerpo (2D12) recomendado para detectarLEF-1 de mouse, rat y human origen, mediante WB y IP

LEF-1 Anticuerpo (2D12): sc-81470

LEF-1 Anticuerpo (2D12) clasificado 4.0 de 5 por 1.
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Ficha técnica

    • LEF-1 Anticuerpo 2D12 es un monoclonal de ratón IgG1, ver las 2 publicaciones, proporcionado como 50 µg/0.5 ml
    • producido contra los amino ácidos 1-85 de recombinant LEF-1 de origen human
    • recomendado para detectarLEF-1 de mouse, rat y human origen, mediante WB y IP
    • Ver LEF-1 (B-10): sc-374412 para LEF-1 anticuerpos conjugados, incluyendo AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 y 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for LEF-1 Antibody (2D12). This work is in progress.
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Could this antibody work for FA fixed chromatin immunoprecipitation?

Preguntado por: Anónimo
Thank you for your question. LEF-1 Antibody (2D12): sc-81470 is only recommended for detection of LEF-1 of mouse, rat and human origin by WB and IP.
Respondida por: Tech Support Europe
Fecha de publicación: 2020-08-25

I am using LEF-1 (2D12): sc-81470 antibody for immunoprecipitation, how much antibody should I use?

Preguntado por: Cweed
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Respondida por: Technical Support
Fecha de publicación: 2017-03-24
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Rated 4 de 5 por de Positive results in western blot using human LEF Positive results in western blot using human LEF-1 transfected lysates. -SCBT QC
Fecha de publicación: 2014-02-26
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