L-type Ca++ CP γ1 CRISPR/Cas9 KO Plasmid (m): sc-419413
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- L-type Ca++ CP γ1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the L-type Ca++ CP γ1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
L-type Ca++ CP γ1 CRISPR/Cas9 KO Plasmid (m) | sc-419413 | 20 µg | $397.00 |
Overview
Cacng1 encodes the L-type Ca++ channel γ1 auxiliary subunit, a tetraspan membrane protein that modulates biophysical properties and membrane expression of voltage-gated calcium channel complexes in excitable tissues. By tuning Ca++ influx, it influences excitation–contraction coupling, activity-dependent signaling, and downstream Ca++-regulated pathways that shape muscle physiology and cellular excitability. Altered regulation of L-type Ca++ channel components has been linked to perturbations in calcium homeostasis associated with neuromuscular and cardiac functional phenotypes, making Cacng1 a useful locus for dissecting channel auxiliary subunit biology in mouse models. Functional studies often interrogate how γ subunits affect channel gating, trafficking, and coupling to Ca++-dependent transcriptional responses.
L-type Ca++ CP γ1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cacng1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cacng1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cacng1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish L-type Ca++ CP γ1 protein expression.
This CRISPR knockout system enables efficient generation of Cacng1-deficient cell models for investigation of L-type Ca++ CP γ1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cacng1 exon(s) critical for L-type Ca++ CP γ1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cacng1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by L-type Ca++ CP γ1 CRISPR/Cas9 KO Plasmid (m) and L-type Ca++ CP γ1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cacng1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by L-type Ca++ CP γ1 HDR Plasmid (m) and L-type Ca++ CP γ1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cacng1 homology arms to support homology-directed repair at defined Cacng1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.