KV3.1 CRISPR/Cas9 KO Plasmid (h): sc-406541
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KV3.1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KV3.1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: KV3.1 Antibody (E-2): sc-514554
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KV3.1 CRISPR/Cas9 KO Plasmid (h) | sc-406541 | 20 µg | $397.00 |
Overview
KCNC1 encodes the voltage-gated potassium channel subunit KV3.1, a fast-activating and rapidly deactivating K⁺ conductance that supports brief action potentials and high-frequency firing in excitable cells. KV3.1 shapes membrane repolarization and firing precision, influencing network oscillations and synaptic timing through regulation of neuronal excitability. KCNC1 activity intersects with broader ion channel signaling processes that coordinate calcium influx, neurotransmitter release probability, and spike-frequency adaptation. Genetic variation or dysregulation of KCNC1 has been associated with neurological phenotypes involving altered excitability, making it relevant for mechanistic studies of channelopathies and circuit dysfunction.
KV3.1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KCNC1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KCNC1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KCNC1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KV3.1 protein expression.
This CRISPR knockout system enables efficient generation of KCNC1-deficient cell models for investigation of KV3.1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KCNC1 exon(s) critical for KV3.1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KCNC1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KV3.1 CRISPR/Cas9 KO Plasmid (h) and KV3.1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KCNC1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KV3.1 HDR Plasmid (h) and KV3.1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KCNC1 homology arms to support homology-directed repair at defined KCNC1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.