Integrin β4/ITGB4/CD104 Antibody (B-7): sc-514426

Integrin β4 Antibody (B-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects Integrin β4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Integrin β4 antibody (B-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Integrin β4, also known as CD104, is a crucial component of the integrin family, functioning primarily as a receptor for Laminin, which is essential for cell adhesion and signaling in epithelial tissues. This 1,822 amino acid single-pass type I membrane protein is predominantly expressed in epithelial cells and plays a vital role in maintaining the integrity of the epithelial barrier. Integrin β4′s ability to interact with Integrin α6 forms a heterodimer that is critical for cellular processes such as migration, proliferation, and differentiation. Additionally, Integrin β4 exists in five alternatively spliced isoforms, which adds complexity to its functional roles in various biological contexts. The gene encoding Integrin β4 is located on human chromosome 17q25.1, highlighting its evolutionary significance and potential implications in developmental biology and disease states.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Integrin β4/ITGB4/CD104 Antibody (B-7) References:

1. Caspase proteolysis of the integrin beta4 subunit disrupts hemidesmosome assembly, promotes apoptosis, and inhibits cell migration.  |  Werner, ME., et al. 2007. J Biol Chem. 282: 5560-9. PMID: 17178732
2. Critical role for integrin-β4 in the attenuation of murine acute lung injury by simvastatin.  |  Chen, W., et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303: L279-85. PMID: 22683568
3. Phosphorylation and nuclear translocation of integrin β4 induced by a chemical small molecule contribute to apoptosis in vascular endothelial cells.  |  Ge, D., et al. 2013. Apoptosis. 18: 1120-31. PMID: 23677256
4. Structural insights into Ca2+-calmodulin regulation of Plectin 1a-integrin β4 interaction in hemidesmosomes.  |  Song, JG., et al. 2015. Structure. 23: 558-570. PMID: 25703379
5. Curcumin Prevents Palmitoylation of Integrin β4 in Breast Cancer Cells.  |  Coleman, DT., et al. 2015. PLoS One. 10: e0125399. PMID: 25938910
6. PKD2 and RSK1 Regulate Integrin β4 Phosphorylation at Threonine 1736.  |  Te Molder, L. and Sonnenberg, A. 2015. PLoS One. 10: e0143357. PMID: 26580203
7. Integrin β4 is a controversial target for non-small cell lung cancer-reply.  |  Stewart, RL. and O'Connor, KL. 2017. Hum Pathol. 61: 223-224. PMID: 27816722
8. Integrin-β4 identifies cancer stem cell-enriched populations of partially mesenchymal carcinoma cells.  |  Bierie, B., et al. 2017. Proc Natl Acad Sci U S A. 114: E2337-E2346. PMID: 28270621
9. Significant Role of Collagen XVII And Integrin β4 in Migration and Invasion of The Less Aggressive Squamous Cell Carcinoma Cells.  |  Moilanen, JM., et al. 2017. Sci Rep. 7: 45057. PMID: 28327550
10. DNA methylation down-regulates integrin β4 expression in asthmatic airway epithelial cells.  |  Wu, M., et al. 2020. Clin Exp Allergy. 50: 1127-1139. PMID: 32618381

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Integrin β4/ITGB4/CD104 Antibody (B-7) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-Integrin β4/ITGB4/CD104 Antibody (B-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Integrin β4/ITGB4/CD104 Antibody (B-7) Specifications

Alternate Product Name: Integrin β4

Product Clone Name: B-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1797-1822

Epitope Target: Integrin β4

Epitope Species: human

Product Reactivity/Detection: Integrin β4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for Integrin β4/ITGB4/CD104 Antibody (B-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Integrin β4/ITGB4/CD104 antibody is Integrin β4/ITGB4/CD104 Antibody (A9): sc-13543.