Integrin α3/ITGA3/CD49c Double Nickase Plasmid (m): sc-421161-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin α3/ITGA3/CD49c Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin α3/ITGA3/CD49c Double Nickase Plasmid (m) and Integrin α3/ITGA3/CD49c Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Itga3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin α3/ITGA3/CD49c Antibody (A-3): sc-374242
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin α3/ITGA3/CD49c Double Nickase Plasmid (m) | sc-421161-NIC | 20 µg | $410.00 | |||
Integrin α3/ITGA3/CD49c Double Nickase Plasmid (m2) | sc-421161-NIC-2 | 20 µg | $410.00 |
Itga3 encodes integrin α3 (CD49c), which heterodimerizes with integrin β1 to form a laminin-binding receptor that mediates cell–extracellular matrix adhesion and basement membrane organization. ITGA3-dependent signaling regulates focal adhesion dynamics, cytoskeletal remodeling, and epithelial cell polarity through pathways that include FAK/Src, PI3K–AKT, and MAPK, influencing migration, proliferation, and survival. In mouse tissues, integrin α3 contributes to epithelial integrity in organs such as skin, lung, and kidney, and its dysregulation is frequently studied in contexts of altered adhesion, inflammation, and invasive phenotypes relevant to cancer biology and tissue remodeling.
Integrin α3/ITGA3/CD49c Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Itga3 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Itga3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Itga3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Itga3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.