insulin R beta Anticuerpo (C-4) recomendado para detectarinsulin Rβ de mouse, rat y human origen, mediante WB, IP, IF y ELISA

insulin Rβ Anticuerpo (C-4): sc-373975

insulin Rβ Anticuerpo (C-4) clasificado 4.7 de 5 por 6.
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Ficha técnica

    • insulin Rβ Anticuerpo (C-4) es un monoclonal de ratón IgG2a κ, ver las 3 publicaciones, proporcionado como 200 µg/ml
    • producido contra los amino ácidos 941-1010 de insulin Rβ de origen human
    • insulin R beta Anticuerpo (C-4) es recomendado para detectar insulin Rβ de mouse, rat y human origen, mediante WB, IP, IF y ELISA; también reactivo con otras especies, incluyendo anyd equine, canine and porcine
    • insulin R beta Anticuerpo (C-4) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de insulin Rβ (C-4): sc-373975.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for insulin Rβ Antibody (C-4) for WB applications. This reagent is now offered in a bundle with insulin Rβ Antibody (C-4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

What is the reason that this antibody recognises ~130 kDa protein, while other Santa Cruz antibodies vs. Insulin Receptor beta reveal a band around ~95 kDa?

Preguntado por: JulP
Thank you for your question. The 130 kDa processed insulin Rβ as detected by insulin Rβ Antibody (C-4): sc-373975 is described in PMID 2187866 “Processed 130-kDa alpha and 95-kDa beta subunits were also observed and contained approximately 20% as much protein as the proreceptor on a molar basis.” Please contact technical service for more information.
Respondida por: SCBT Heidelberg Support
Fecha de publicación: 2019-01-06

What is the recommended starting dilution for Western blot for sc-373975: insulin Rβ (C-4) monoclonal antibody?

Preguntado por: AbPolly
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Respondida por: Technical Support
Fecha de publicación: 2017-03-22
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Rated 5 de 5 por de Excellent I brought this product and i got excellent results. also the customer service and tech support are helpful
Fecha de publicación: 2019-12-11
Rated 4 de 5 por de No positivity in S. aurata and D. labrax tissues At the tested dilution (1:50, 1:200) and with the protocol in use in our laboratories, it was not possible to detect any positivity in Sparus aurata and Dicentrarchus labrax tissues
Fecha de publicación: 2019-07-03
Rated 4 de 5 por de IF on mouse mammary cancer cell Antibody – Santa Cruz 373975 anti-Insulin receptor beta Cell – mouse mammary cancer cell IF tested – dilution 1:50
Fecha de publicación: 2017-12-18
Rated 5 de 5 por de Excellent antibody The insulin R beta Antibody (C-4): sc-373975 works very well for detection of Insulin R via Western blot, showing strong and clean signal.
Fecha de publicación: 2017-02-09
Rated 5 de 5 por de Works well for WB in HeLa and JAR whole cell Works well for WB in HeLa and JAR whole cell lysates. -SCBT QC
Fecha de publicación: 2015-01-17
Rated 5 de 5 por de Strong band observed in western blot analysis Strong band observed in western blot analysis of insulin R expression in Hep G2 whole cell lysate. -SCBT QC
Fecha de publicación: 2013-12-04
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