IL-3Rα CRISPR/Cas9 KO Plasmid (m): sc-421109
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-3Rα CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IL-3Rα genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-3Rα Antibody (C-5): sc-74522
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-3Rα CRISPR/Cas9 KO Plasmid (m) | sc-421109 | 20 µg | $397.00 |
Overview
Il3ra encodes the alpha chain of the interleukin-3 receptor (IL-3Rα, CD123), which confers ligand-binding specificity and forms a signaling complex with the common beta chain to transduce IL-3–dependent cues. In mouse hematopoietic and immune cells, IL-3Rα regulates proliferation, survival, and differentiation, engaging downstream JAK/STAT, PI3K/AKT, and MAPK pathways that shape myeloid lineage responses. Altered IL-3Rα signaling can perturb cytokine-driven homeostasis and inflammatory programs, making Il3ra a useful target for studying hematopoiesis, myeloid activation states, and cytokine receptor cross-talk. The receptor is also widely used as an immunophenotyping marker to interrogate development and functional heterogeneity across hematopoietic compartments.
IL-3Rα CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Il3ra gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Il3ra together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Il3ra open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IL-3Rα protein expression.
This CRISPR knockout system enables efficient generation of Il3ra-deficient cell models for investigation of IL-3Rα signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Il3ra exon(s) critical for IL-3Rα function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Il3ra genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IL-3Rα CRISPR/Cas9 KO Plasmid (m) and IL-3Rα CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Il3ra locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IL-3Rα HDR Plasmid (m) and IL-3Rα HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Il3ra homology arms to support homology-directed repair at defined Il3ra target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.