IL-10 Double Nickase Plasmid (m): sc-421076-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-10 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- IL-10 Double Nickase Plasmid (m) and IL-10 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Il10. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-10 Antibody (A-2): sc-365858
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-10 Double Nickase Plasmid (m) | sc-421076-NIC | 20 µg | $410.00 |
Mouse Il10 encodes interleukin-10 (IL-10), an immunoregulatory cytokine produced by multiple leukocyte subsets that constrains innate and adaptive inflammatory programs. IL-10 signals through the IL-10 receptor complex to activate JAK1/TYK2-dependent STAT3 transcriptional responses, dampening NF-κB–driven cytokine production, antigen presentation, and co-stimulatory pathways. This axis shapes macrophage and dendritic cell polarization, limits Th1/Th17 effector activity, and supports tissue homeostasis at mucosal barriers. Dysregulated IL-10 signaling is linked to aberrant inflammatory responses and altered susceptibility to immune-mediated pathology, making Il10 a key node for studying cytokine feedback control in disease-relevant models.
IL-10 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Il10 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Il10. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Il10 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Il10-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.