Hugl-1 Antibody (B-6): sc-136993

Hugl-1 Antibody (B-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects Hugl-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Hugl-1 (B-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Hugl-1 plays a crucial role in regulating mitotic spindle orientation, differentiation, proliferation, and tissue organization of neuroepithelial cells, highlighting its importance in maintaining proper cellular architecture and function. Localized primarily in the cytoplasm, Hugl-1 is part of a complex essential for preserving cell polarity and epithelial integrity, which are vital for normal tissue development and function. Additionally, Hugl-1 interacts with several key proteins, including nonmuscle Myosin II heavy chain, PRKCI/aPKC, PARD6B/Par-6, PARD6A, and STX4A, further underscoring its role in cellular signaling pathways. Hugl-1 expression is observed in tissues such as the kidney, brain, and muscle, where Hugl-1 enhances cell adhesion and reduces cell migration. As a tumor suppressor in humans, loss of Hugl-1 expression has been implicated in the progression of colorectal cancer and melanoma. LLGL1, the gene encoding Hugl-1, shares significant homology with the Drosophila tumor suppressor gene l(2)gl, which encodes Lgl, also involved in maintaining cell polarity and epithelial integrity.

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Hugl-1 Antibody (B-6) References:

1. Mammalian Lgl forms a protein complex with PAR-6 and aPKC independently of PAR-3 to regulate epithelial cell polarity.  |  Yamanaka, T., et al. 2003. Curr Biol. 13: 734-43. PMID: 12725730
2. The human protein Hugl-1 substitutes for Drosophila lethal giant larvae tumour suppressor function in vivo.  |  Grifoni, D., et al. 2004. Oncogene. 23: 8688-94. PMID: 15467749
3. Reduced expression of Hugl-1, the human homologue of Drosophila tumour suppressor gene lgl, contributes to progression of colorectal cancer.  |  Schimanski, CC., et al. 2005. Oncogene. 24: 3100-9. PMID: 15735678
4. Molecular requirements for epithelial-mesenchymal transition during tumor progression.  |  Huber, MA., et al. 2005. Curr Opin Cell Biol. 17: 548-58. PMID: 16098727
5. Expression of Hugl-1 is strongly reduced in malignant melanoma.  |  Kuphal, S., et al. 2006. Oncogene. 25: 103-10. PMID: 16170365
6. Aberrant splicing of Hugl-1 is associated with hepatocellular carcinoma progression.  |  Lu, X., et al. 2009. Clin Cancer Res. 15: 3287-96. PMID: 19447873
7. Preservation of HUGL-1 expression as a favourable prognostic factor in pancreatic carcinoma.  |  Biesterfeld, S., et al. 2012. Anticancer Res. 32: 3153-9. PMID: 22843887
8. Hugl-1 induces apoptosis in esophageal carcinoma cells both in vitro and in vivo.  |  Song, J., et al. 2013. World J Gastroenterol. 19: 4127-36. PMID: 23864775
9. Hugl-1 inhibits glioma cell growth in intracranial model.  |  Liu, X., et al. 2015. J Neurooncol. 125: 113-21. PMID: 26341367
10. Reduced Expression of Hugl 1 Contributes to the Progression of Lung Squamous Cell Carcinoma.  |  Matsuzaki, T., et al. 2015. Tokai J Exp Clin Med. 40: 169-77. PMID: 26662669
11. A human homologue of the Drosophila tumour suppressor gene l(2)gl maps to 17p11.2-12 and codes for a cytoskeletal protein that associates with nonmuscle myosin II heavy chain.  |  Strand, D., et al. 1995. Oncogene. 11: 291-301. PMID: 7542763
12. The human homologue of the murine Llglh gene (LLGL) maps within the Smith-Magenis syndrome region in 17p11.2.  |  Koyama, K., et al. 1996. Cytogenet Cell Genet. 72: 78-82. PMID: 8565641

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Hugl-1 Antibody (B-6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Hugl-1 Antibody (B-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Hugl-1 Antibody (B-6) Specifications

Alternate Product Name: Hugl-1

Product Clone Name: B-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 937-1062

Epitope Target: Hugl-1

Epitope Species: human

Product Reactivity/Detection: Hugl-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Hugl-1 Antibody (B-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin