Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HSP 27 Double Nickase Plasmid (h): sc-400285-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSP 27 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HSP 27 Double Nickase Plasmid (h) and HSP 27 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HSPB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HSP 27 Antibody (F-4): sc-13132
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSP 27 Double Nickase Plasmid (h) | sc-400285-NIC | 20 µg | $410.00 | |||
HSP 27 Double Nickase Plasmid (h2) | sc-400285-NIC-2 | 20 µg | $410.00 |
HSPB1 encodes the small heat shock protein HSP 27, an ATP-independent molecular chaperone that stabilizes unfolded proteins, supports proteostasis, and modulates cytoskeletal dynamics through interactions with actin and intermediate filaments. HSP 27 is induced by cellular stress via heat shock factor signaling and participates in oxidative stress responses, regulation of apoptosis, and control of protein aggregation. Through these functions, HSPB1 influences pathways linked to inflammation, cell survival, and stress-adaptive remodeling, making it relevant to studies of neurodegeneration, cardiovascular stress injury, and cancer cell stress tolerance. Altered HSPB1 expression or phosphorylation state has been associated with changes in proteome stability and stress signaling outputs in multiple disease contexts.
HSP 27 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HSPB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HSPB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HSPB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HSPB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.