Hmx3 CRISPR/Cas9 KO Plasmid (m): sc-420887
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Hmx3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Hmx3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Hmx3 CRISPR/Cas9 KO Plasmid (m) | sc-420887 | 20 µg | $397.00 |
Overview
Hmx3 (homeobox protein Hmx3) is a transcription factor that regulates developmental gene expression programs through sequence-specific DNA binding and control of cell fate decisions. In mouse, Hmx3 activity is closely linked to inner ear morphogenesis and neurodevelopmental patterning, coordinating downstream transcriptional networks that shape sensory structures and neuronal differentiation. Disruption of homeobox-driven regulatory circuits involving Hmx3 can alter embryonic patterning and organogenesis, making it relevant for studying congenital developmental phenotypes and the gene regulatory logic of sensory system formation. As a nuclear regulator, Hmx3 is often investigated in the context of transcriptional control, lineage specification, and developmental signaling cross-talk.
Hmx3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hmx3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hmx3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hmx3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Hmx3 protein expression.
This CRISPR knockout system enables efficient generation of Hmx3-deficient cell models for investigation of Hmx3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hmx3 exon(s) critical for Hmx3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hmx3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Hmx3 CRISPR/Cas9 KO Plasmid (m) and Hmx3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hmx3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Hmx3 HDR Plasmid (m) and Hmx3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hmx3 homology arms to support homology-directed repair at defined Hmx3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.