GPR78 CRISPR/Cas9 KO Plasmid (h): sc-405837
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR78 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GPR78 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR78 CRISPR/Cas9 KO Plasmid (h) | sc-405837 | 20 µg | $397.00 |
Overview
GPR78 encodes a G protein-coupled receptor implicated in transmembrane signal transduction and coordination of cellular responses to extracellular cues. As a GPCR, GPR78 is positioned to modulate downstream second messenger pathways, including cAMP- and calcium-linked signaling, with potential effects on cell proliferation, survival, and motility programs. Altered GPCR expression or signaling dynamics are frequently associated with dysregulated tissue homeostasis and oncogenic processes, making GPR78 relevant for mechanistic studies in cancer biology and related signaling phenotypes. Characterizing GPR78 function also supports pathway mapping efforts that connect receptor activity to transcriptional and metabolic adaptations.
GPR78 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GPR78 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GPR78 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GPR78 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GPR78 protein expression.
This CRISPR knockout system enables efficient generation of GPR78-deficient cell models for investigation of GPR78 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GPR78 exon(s) critical for GPR78 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GPR78 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GPR78 CRISPR/Cas9 KO Plasmid (h) and GPR78 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GPR78 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GPR78 HDR Plasmid (h) and GPR78 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GPR78 homology arms to support homology-directed repair at defined GPR78 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.