GPR39 Double Nickase Plasmid (h): sc-410802-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR39 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GPR39 Double Nickase Plasmid (h) and GPR39 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GPR39. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR39 Double Nickase Plasmid (h) | sc-410802-NIC | 20 µg | $410.00 | |||
GPR39 Double Nickase Plasmid (h2) | sc-410802-NIC-2 | 20 µg | $410.00 |
GPR39 encodes a zinc-activated G protein-coupled receptor predominantly linked to Gαq/11 and Gαs signaling, integrating extracellular cues into second-messenger responses such as phospholipase C activation, intracellular Ca2+ mobilization, and cAMP production. Through these pathways, GPR39 can influence transcriptional programs that regulate cell survival, proliferation, epithelial and endothelial barrier function, and metabolic homeostasis. The receptor has been studied in contexts including gastrointestinal physiology, inflammatory signaling, and neuroendocrine regulation, where altered GPCR signaling dynamics can reshape tissue responses to stress. Dysregulated GPR39-associated signaling has been investigated across multiple disease-relevant processes, making it a useful target for mechanistic studies of zinc sensing, GPCR desensitization, and downstream kinase cascades.
GPR39 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GPR39 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GPR39. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GPR39 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GPR39-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.