Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GlyR β Double Nickase Plasmid (h): sc-403759-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GlyR β Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GlyR β Double Nickase Plasmid (h) and GlyR β Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GLRB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GlyR β Antibody (D-8): sc-365819
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GlyR β Double Nickase Plasmid (h) | sc-403759-NIC | 20 µg | $410.00 | |||
GlyR β Double Nickase Plasmid (h2) | sc-403759-NIC-2 | 20 µg | $410.00 |
GLRB encodes the glycine receptor beta subunit (GlyR β), an essential component of pentameric ligand-gated chloride channels that mediate fast inhibitory neurotransmission in the spinal cord and brainstem. GlyR β contributes to receptor assembly, synaptic localization, and channel gating, linking glycinergic signaling to membrane hyperpolarization and regulation of neuronal excitability. Through coordination with scaffolding proteins such as gephyrin, GLRB supports inhibitory synapse organization and chloride homeostasis. Genetic and functional perturbations of GLRB are associated with disrupted inhibitory signaling and neurological phenotypes relevant to startle disease and other disorders of motor control.
GlyR β Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GLRB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GLRB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GLRB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GLRB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.