Glucose Transporter Glut8 Double Nickase Plasmid (m): sc-424976-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Glucose Transporter Glut8 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Glucose Transporter Glut8 Double Nickase Plasmid (m) and Glucose Transporter Glut8 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Slc2a8. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Glucose Transporter Glut8 Double Nickase Plasmid (m) | sc-424976-NIC | 20 µg | $410.00 |
Slc2a8 encodes glucose transporter GLUT8 (SLC2A8), a facilitative hexose transporter that contributes to cellular carbohydrate uptake and intracellular glucose handling in mouse tissues. GLUT8 activity influences energy homeostasis by modulating glycolytic flux and substrate availability for oxidative metabolism, intersecting with insulin-responsive glucose utilization and nutrient-sensing pathways such as AMPK and mTOR. In addition to systemic metabolism, SLC2A8 has been linked to reproductive and neuronal physiology where altered glucose transport can impact cellular viability and differentiation. Dysregulated glucose transport and metabolic reprogramming are broadly relevant to obesity, insulin resistance, and diabetes-related mechanisms, supporting investigation of Slc2a8 in metabolic stress and tissue-specific glucose handling.
Glucose Transporter Glut8 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Slc2a8 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Slc2a8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Slc2a8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Slc2a8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.