G alpha i-1 Anticuerpo (R4) recomendado para detectarGα i-1 de mouse, rat, human y bovine origen, mediante WB, IP, IF y IHC(P)

Gα i-1 Anticuerpo (R4): sc-13533

Gα i-1 Anticuerpo (R4) clasificado 4.6 de 5 por 12.
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Ficha técnica

    • Gα i-1 Anticuerpo (R4) es un monoclonal de ratón IgG2b κ, ver las 25 publicaciones, proporcionado como 200 µg/ml
    • producido contra Gα i-1 de origen rat
    • G alpha i-1 Anticuerpo (R4) es recomendado para detectar Gα i-1 de mouse, rat, human y bovine origen, mediante WB, IP, IF y IHC(P)
    • G alpha i-1 Anticuerpo (R4) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de Gα i-1 (R4): sc-13533.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Gα i-1 Antibody (R4) for WB and IHC(P) applications. This reagent is now offered in a bundle with Gα i-1 Antibody (R4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Is sc-13533 (Gα i-1 -R4-) tested for cross-reactivity with Gα0 and Gα-i 2 & 3 isoforms

Preguntado por: Miguelon
Thank you for your question. sc-13533 is specific for Gα i-1, and should not cross-react with any other Gα forms.
Respondida por: Tech Service
Fecha de publicación: 2020-02-11

In which region of Gα i-1 does the antibody for Gα i-1 (R4): sc-13533 recognize (epitope region)?

Preguntado por: Alex2323
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Respondida por: Technical Support
Fecha de publicación: 2018-12-21

Can Gα i-1 (R4): sc-13533 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Preguntado por: jenni
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Gα i-1 (R4): sc-13533 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Respondida por: Technical Support
Fecha de publicación: 2017-02-24
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Rated 4 de 5 por de Works for IF I used this for IF staining brain sections and it works well
Fecha de publicación: 2017-08-15
Rated 5 de 5 por de Good operation This antibody is suitable for many cell lines, I used it to detect Gα i-1, which was derived from WB mouse, and achieved good results.
Fecha de publicación: 2017-05-23
Rated 5 de 5 por de The catalyst The test catalyst, adenylate cyclase, each of a very broad range of receptors specifically detects extracellular stimuli, whereas the effectors used to generate one or more intracellular messengers are less
Fecha de publicación: 2017-02-09
Rated 5 de 5 por de Very popular choice for WB! This monoclonal G alpha i-1 Antibody (R4) is an excellent choice for Western blotting, IF and IHC(P). Published over a dozen times, especially popular in the literature for Western blotting applications.
Fecha de publicación: 2017-02-07
Rated 5 de 5 por de Recommended use ublished data for IP and WB in mouse melanocytes,great western blot results,strong signal with low background,also nice IHC data.
Fecha de publicación: 2017-02-07
Rated 5 de 5 por de Great antibody for WB This antibody is really great. In the experiment, the analysis results of Western blot were very strong and clear, and the baseband is perfect.antibody diluted at 1:400.
Fecha de publicación: 2017-01-26
Rated 5 de 5 por de reliable It worked for me from the first attempt with a 1:1000 dilution, this has worked very well in WB, it is predictable and reliable.
Fecha de publicación: 2017-01-12
Rated 5 de 5 por de Great antibody I thought it was a great antibody. It helped me make a good progress of my project. I am quite willing to continue to use this antibody.
Fecha de publicación: 2017-01-12
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