GalNAc-TL5 Antibody (F-5): sc-398569

GalNAc-TL5 Antibody (F-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects GalNAc-TL5 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-GalNAc-TL5 antibody (F-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GalNAc-TL5, also known as GALNTL5, is a crucial member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase family, which plays a vital role in initiating mucin-type O-linked glycosylation within the Golgi apparatus. This 443 amino acid single-pass type II membrane protein is specifically localized to the Golgi apparatus and is expressed predominantly in the testis, where GalNAc-TL5 utilizes manganese and calcium as essential cofactors for enzymatic activity. GalNAc-TL5 is responsible for transferring an N-acetyl-D-galactosamine residue to serine or threonine residues on protein receptors, catalyzing the initial step in the biosynthesis of O-linked oligosaccharides. Unlike other members of the GalNAc-T subfamily, GalNAc-TL5 lacks a C-terminal ricin B-type lectin domain, which may influence functional specificity. GalNAc-TL5 features two conserved domains within its glycosyltransferase region: the N-terminal domain, known as domain A or GT1 motif, is likely involved in manganese coordination and substrate binding, while the C-terminal domain, referred to as domain B or Gal/GalNAc-T motif, is thought to play a role in catalytic reactions and UDP-Gal binding. GalNAc-TL5 exists in two alternatively spliced isoforms, further contributing to functional diversity.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GalNAc-TL5 Antibody (F-5) References:

1. Cloning and characterization of a close homologue of human UDP-N-acetyl-alpha-D-galactosamine:Polypeptide N-acetylgalactosaminyltransferase-T3, designated GalNAc-T6. Evidence for genetic but not functional redundancy.  |  Bennett, EP., et al. 1999. J Biol Chem. 274: 25362-70. PMID: 10464263
2. Activity of partially purified UDP-N-acetyl-alpha-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase with different peptide acceptors.  |  Porowska, H., et al. 1999. Acta Biochim Pol. 46: 365-70. PMID: 10547037
3. Intact Golgi synthesize complex branched O-linked chains on glycoside primers: evidence for the functional continuity of seven glycosyltransferases and three sugar nucleotide transporters.  |  Kim, S., et al. 2001. Glycoconj J. 18: 623-33. PMID: 12376727
4. The DNA sequence of human chromosome 7.  |  Hillier, LW., et al. 2003. Nature. 424: 157-64. PMID: 12853948
5. Differing reactions of monoclonal anti-A antibodies with oligosaccharides related to blood group A.  |  Gooi, HC., et al. 1985. J Biol Chem. 260: 13218-24. PMID: 2414277
6. The biosynthesis of oligosaccharides in intact Golgi preparations from rat liver. Analysis of N-linked and O-linked glycans labeled by UDP-[6-3H]N-acetylgalactosamine.  |  Hayes, BK. and Varki, A. 1993. J Biol Chem. 268: 16170-8. PMID: 8344901

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GalNAc-TL5 Antibody (F-5) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-GalNAc-TL5 Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GalNAc-TL5 Antibody (F-5) Specifications

Alternate Product Name: GalNAc-TL5

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 238-284

Epitope Target: GalNAc-TL5

Epitope Species: mouse

Product Reactivity/Detection: GalNAc-TL5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for GalNAc-TL5 Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin