GAL4-TA Antibody (D-4): sc-48399
- GAL4-TA Antibody (D-4) is a mouse monoclonal IgG3, cited in 1 publications, provided at 200 µg/ml
- raised against GAL4 -TA corresponding to amino acids 768-881 of yeast origin
- recommended for detection of GAL4-TA by WB, IP, IF and ELISA
- See GAL4-TA (A-2): sc-46680 for GAL4-TA antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for GAL4-TA Antibody (D-4). This work is in progress.
QUICK LINKS
GAL4-TA Antibody (D-4) is a mouse monoclonal IgG3 antibody that detects GAL4 by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GAL4 (D-4) antibody is essential for studying GAL4 function, one of the most extensively characterized transcriptional activators. GAL4 plays a crucial role in regulating the expression of genes involved in galactose metabolism, specifically activating GAL genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 in response to galactose. The unique structure of GAL4, particularly its N-terminal 147 amino acid residues, allows for specific and strong binding to DNA, while the C-terminal transcriptional activation domain, spanning amino acids 768-881, is responsible for the activation of these genes. This structural distinction enables researchers to utilize GAL4 in various experimental systems, including yeast, plants, insects, and mammalian cells, to investigate transcriptional activation functions and protein-protein interactions. GAL4 (D-4) monoclonal antibody is particularly valuable in the development of the "two-hybrid" system, which leverages GAL4 fusions to identify specific interactions between proteins, thereby advancing our understanding of cellular processes and regulatory mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GAL4-TA Antibody (D-4) References:
- The 9aaTAD Transactivation Domains: From Gal4 to p53. | Piskacek, M., et al. 2016. PLoS One. 11: e0162842. PMID: 27618436
- Regulating the UAS/GAL4 system in adult Drosophila with Tet-off GAL80 transgenes. | Barwell, T., et al. 2017. PeerJ. 5: e4167. PMID: 29259847
- Characterization of a morphogenetic furrow specific Gal4 driver in the developing Drosophila eye. | Sarkar, A., et al. 2018. PLoS One. 13: e0196365. PMID: 29702674
- Modeling transcriptional activation changes to Gal4 variants via structure-based computational mutagenesis. | Masso, M., et al. 2018. PeerJ. 6: e4844. PMID: 29868268
- A zinc-finger fusion protein refines Gal4-defined neural circuits. | Raghu, SV., et al. 2018. Mol Brain. 11: 46. PMID: 30126464
- GAL4/GFP enhancer-trap lines for identification and manipulation of cells and tissues in developing Arabidopsis leaves. | Amalraj, B., et al. 2020. Dev Dyn. 249: 1127-1146. PMID: 32319191
- Optimization of Light-Inducible Gal4/UAS Gene Expression System in Mammalian Cells. | Yamada, M., et al. 2020. iScience. 23: 101506. PMID: 32919371
- Molecular and cytological analysis of widely-used Gal4 driver lines for Drosophila neurobiology. | Ogienko, AA., et al. 2020. BMC Genet. 21: 96. PMID: 33092520
- Exploiting the Gal4/UAS System as Plant Orthogonal Molecular Toolbox to Control Reporter Expression in Arabidopsis Protoplasts. | Iacopino, S., et al. 2022. Methods Mol Biol. 2379: 99-111. PMID: 35188658
- A split GAL4 RUBY assay for visual in planta detection of protein-protein interactions. | Chen, J., et al. 2023. Plant J. 114: 1209-1226. PMID: 37323061
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GAL4-TA Antibody (D-4) | sc-48399 | 200 µg/ml | $322.00 |