Date published: 2025-9-7

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FOXM1 Anticuerpo (G-5): sc-376471

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Fichas Técnicas
  • FOXM1 Anticuerpo G-5 es un monoclonal de ratón IgG2b κ FOXM1 Anticuerpo, ver las 64 publicaciones, proporcionado como 200 µg/ml
  • específico para un epítopo localizado entre los amino ácidos 734-763 en el C-terminus de FOXM1 de origen human
  • FOXM1 Anticuerpo (G-5) es recomendado para detectar all isoforms of FOXM1 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA
  • FOXM1 Anticuerpo (G-5) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
  • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
  • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
  • TransCruz reactivo para Gel Supershift y ChIP (sc-376471 X, 200 µg/0.1 ml)
  • Contacte con nosotros para recibir GRATIS 10 µg de muestra de FOXM1 (G-5): sc-376471.
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP y m-IgGκ BP-HRP son los reactivos de detección secundarios preferidos para FOXM1 Anticuerpo (G-5) para aplicactiones WB and IHC(P). Estos reactivos se ofrecen ahora en paquetes con FOXM1 Anticuerpo (G-5) (ver información de pedido más abajo).

ENLACES RÁPIDOS

VER TAMBIÉN ....

El Anticuerpo FOXM1 (G-5) es un anticuerpo monoclonal de ratón IgG2b κ que detecta la proteína FOXM1 de origen ratón, rata y humano por WB, IP, IF, IHC(P) y ELISA. Está disponible en forma no conjugada, así como en varias formas conjugadas, incluyendo agarosa, HRP, PE, FITC y múltiples conjugados de Alexa Fluor®. La familia Fox de factores de transcripción es un grupo grande de proteínas que comparten un dominio de unión al ADN común llamado dominio de winged-helix o forkhead. FOXM1, también conocido como FKHL16, MPP2 o Trident, se expresa principalmente en células en proliferación. La disminución en la expresión de FOXM1 se asocia con defectos relacionados con la edad en la proliferación celular. Por otro lado, un aumento en la expresión de FOXM1B en los hígados de ratones transgénicos mayores restaura las tasas de replicación del ADN de los hepatocitos a la tasa más alta presente en hígados jóvenes. FOXM1B activa la transcripción de ciclina B1, ciclina D1 y Cdc25B.

Para uso exclusivo en Investigación No está diseñado para para Diagnóstico ó Terapia.

Alexa Fluor® es una marca registrada de Molecular Probes Inc., OR., USA

REIVEW LI-COR® y Odyssey® son marcas registradas de LI-COR Biosciences.

Información sobre pedidos

Nombre del productoNúmero de catálogoUNIDADPrecioCANTIDADFavoritos

FOXM1 Anticuerpo (G-5)

sc-376471
200 µg/ml
$316.00

Paquete de FOXM1 (G-5): m-IgG Fc BP-HRP

sc-529873
200 µg Ab; 10 µg BP
$354.00

Paquete de FOXM1 (G-5): m-IgGκ BP-HRP

sc-522963
200 µg Ab, 40 µg BP
$354.00

Paquete de FOXM1 (G-5): m-IgG2b BP-HRP

sc-550146
200 µg Ab; 10 µg BP
$354.00

FOXM1 Anticuerpo (G-5) X

sc-376471 X
200 µg/0.1 ml
$316.00

FOXM1 Anticuerpo (G-5) AC

sc-376471 AC
500 µg/ml, 25% agarose
$416.00

FOXM1 Anticuerpo (G-5) HRP

sc-376471 HRP
200 µg/ml
$316.00

FOXM1 Anticuerpo (G-5) FITC

sc-376471 FITC
200 µg/ml
$330.00

FOXM1 Anticuerpo (G-5) PE

sc-376471 PE
200 µg/ml
$343.00

FOXM1 Anticuerpo (G-5) Alexa Fluor® 488

sc-376471 AF488
200 µg/ml
$357.00

FOXM1 Anticuerpo (G-5) Alexa Fluor® 546

sc-376471 AF546
200 µg/ml
$357.00

FOXM1 Anticuerpo (G-5) Alexa Fluor® 594

sc-376471 AF594
200 µg/ml
$357.00

FOXM1 Anticuerpo (G-5) Alexa Fluor® 647

sc-376471 AF647
200 µg/ml
$357.00

FOXM1 Anticuerpo (G-5) Alexa Fluor® 680

sc-376471 AF680
200 µg/ml
$357.00

FOXM1 Anticuerpo (G-5) Alexa Fluor® 790

sc-376471 AF790
200 µg/ml
$357.00

FOXM1 (G-5) péptido neutralizante

sc-376471 P
100 µg/0.5 ml
$68.00

FOXM1 G-5 is mouse monoclonal IgG2b. I would like to know if it can be used with conventional secondary anti-mouse IgG reagents.

Preguntado por: Gemo
Thank you for your question. Yes, you can use a conventional anti-mouse IgG secondary antibody for detection of this primary antibody. For more specific results, we do recommend using an isotype-specific secondary antibody (anti-mouse IgG2b), or our mouse IgGκ binding protein, e.g., m-IgGκ BP-HRP: sc-516102.
Respondida por: Technical Support
Fecha de publicación: 2020-03-09

Cantidad de anticuerpo FOXM1(G5) X TransCruz sc-376471 recomendado para usar en ChIP.

Preguntado por: femili12
Gracias por sus preguntas. Recomendamos el uso de 100-500 μg de sobrenadante y 0,1-1 μl de reactivo TransCruz (0,2-2 μg). Para obtener más información, haga clic en este enlace de nuestro protocolo ChIP: https://www.scbt.com/scbt/resources/protocols/chromatin-immunoprecipitation-assays Thank you for your questions. We recommend using 100–500 µg supernatant and 0.1-1 µl TransCruz reagent (0.2–2 µg). For further information, please click on this link to our ChIP protocol: https://www.scbt.com/scbt/resources/protocols/chromatin-immunoprecipitation-assays
Respondida por: Technical Support
Fecha de publicación: 2018-12-31

Which type of FOXM1  give good results for IHC on parrafin bock,human tissue breast cancer

Preguntado por: Madyan
Thank you for your question. We would recommend using our mouse monoclonal antibody, FOXM1 (G-5): sc-376471. We have several pieces of IHC(P) data in human tissue using this antibody on our website.
Respondida por: Technical Support
Fecha de publicación: 2017-08-22

Does the FOXM1 Antibody (G-5): sc-376471 detect isoform “C” of the FOXM1 target?

Preguntado por: Trav11
Thank you for your question. The FOXM1 Antibody (G-5): sc-376471 will detect all isoforms (A, B, & C) for the FOXM1 protein target. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Respondida por: Technical Support 15
Fecha de publicación: 2017-01-16

Does FOXM1 Antibody (G-5): sc-376471 react with Zebrafish samples?

Preguntado por: Trav11
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Respondida por: Technical Support 13
Fecha de publicación: 2017-01-13
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Rated 5 de 5 por de 功能不错,敏感度很高,很好跑半年前试用了一周,感觉效果很棒,再次购买发现没让人失望,效果真的很不错,以后还会再买他家的抗体,都可以试试。
Fecha de publicación: 2023-10-11
Rated 4 de 5 por de 经理BACKGROUND The Fox family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXM1, also known as FKHL16, MPP2 or Trident, is primarily expressed in proliferating cells. The gene encoding human FOXM1 maps to chromosome 12p13.33. The transcription element that restricts FOXM1 expression to prolif- erating cells is located 300 bp upstream of the start codon. FOXM1 is most abundant in thymus, testis, small intestine and colon. Alternative splicing generates FOXM1A and FOXM1B isoforms that contain PEST regions involved in rapid protein degradation. A decrease in FOXM1 expression is associated with age-related defects in cellular proliferation. Conversely, an increase in FOXM1B expression in the livers of older transgenic mice restore hepatocyte DNA replication rates to the higher rate present in young livers. FOXM1B activates the transcription of cyclin B1, cyclin D1 and Cdc25B
Fecha de publicación: 2021-06-23
Rated 5 de 5 por de the greatest Ab i ever had!it works very well in both KD and OE systems i set up. believe me, commercial ABs of FOXM1 from other companies dont work at all. this showed clear band, at 1:250 dilution with senescent dermal fibroblast cell samples.
Fecha de publicación: 2020-07-22
Rated 1 de 5 por de Not specific for FOXM1 - Do not use this antibody!As noticed by other users before, the FOXM1 G-5 antibody detects a protein with the same molecular weight as FOXM1 in Western Blots, but
Fecha de publicación: 2018-07-18
Rated 4 de 5 por de Great antibodiesI got a free sample from company. I used antibodies in WB in dilution 1:1000. It works good.
Fecha de publicación: 2018-07-14
Rated 1 de 5 por de Not sure if observed band is FOXM1Like the previous comments below, our group has also confirmed that this antibody may not be recognizing FOXM1. We observe a strong clear band at around 125-130 kDa with this antibody in our cell lines. However, upon using both siRNA and shRNA approaches with 3 different species, we could not observe a clear knockdown of FOXM1 with this antibody. In contrast, FOXM1 (A-11) sc-271746 showed knockdown of FOXM1 protein. Additionally, while this antibody can recognize overexpressed FOXM1, the exogenous FOXM1 appears as a second at around 110 kDa. The FOXM1 (A-11) antibody only recognizes a single band with much greater intensity. The FOXM1 (A-11) antibody also gave consistent results with CS #D12D5 and an Abcam antibody.
Fecha de publicación: 2018-05-11
Rated 3 de 5 por de Gives good band, but uncertain about specificityThe mouse G-5 antibody appears to detect primarily nuclear FOXM1; whereas the SC-500 rabbit antibody detects mainly cytoplasmic (and to some extent nuclear). The expression pattern in western blots, ICC, and IHC have confirmed this (images attached). Possibly due to the G-5 antibody unable to detect FOXM1 in cytoplasm if dimerized? It seems to detect FOXM1 differently in regards to localization; however, the MW appears to be correct. I still haven't quite figured this one out. In any case, I used the antibody at a 1:1000 dilution and secondary at 1:2500. I've noticed most SC antibodies work well at 1:250/500 primary and secondary at 1:2500. It has worked well with overexpression (human FOXM1b isoform) as well as siRNA knockdown in rat cells.
Fecha de publicación: 2018-03-26
Rated 1 de 5 por de Not sure the band we detect is FOXM1We have observed similar results than the one showed by elenaenzo with two different siRNA against FOXM1 in HeLa cells (an antibody against FOXM1 from another firm was used as a positive control on the same lysates).
Fecha de publicación: 2018-03-16
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FOXM1 Anticuerpo (G-5) clasificado 4.0 de 5 por 28.
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