FBPase Antibody (C-10): sc-166145
- FBPase Antibody (C-10) is a mouse monoclonal IgG2b κ FBPase antibody provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of liver FBPase of human origin
- FBPase Antibody (C-10) is recommended for detection of liver and muscle FBPase of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-FBPase Antibody (C-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for FBPase Antibody (C-10) for WB and IHC(P) applications. These reagents are now offered in bundles with FBPase Antibody (C-10) (see ordering information below).
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FBPase Antibody (C-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects FBPase protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-FBPase antibody (C-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Fructose-1,6-bisphosphatase (FBPase) plays a crucial role in carbohydrate metabolism by catalyzing the conversion of fructose-1,6-bisphosphate into fructose 6-phosphate and inorganic phosphate, a key step in gluconeogenesis that helps maintain blood glucose levels during fasting. The enzyme is encoded by two genes, FBP1 and FBP2, which produce liver and muscle isoforms, respectively, and is found in all living organisms, highlighting its fundamental importance in energy metabolism. The liver isoform of FBPase is composed of four identical subunits, and AMP regulates enzymatic activity by affecting the turnover of bound substrate rather than its affinity. Mutations in the FBP1 gene can lead to an autosomal recessive disorder characterized by FBPase deficiency, resulting in hypoglycemia and metabolic acidosis, while the muscle isoform is strategically located on both sides of the Z-line, facilitating its role in muscle metabolism.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
FBPase Antibody (C-10) References:
- Two newly identified genomic mutations in a Japanese female patient with fructose-1,6-bisphosphatase (FBPase) deficiency. | Matsuura, T., et al. 2002. Mol Genet Metab. 76: 207-10. PMID: 12126934
- Different sensitivities of mutants and chimeric forms of human muscle and liver fructose-1,6-bisphosphatases towards AMP. | Rakus, D., et al. 2003. Biol Chem. 384: 51-8. PMID: 12674499
- Interaction between muscle aldolase and muscle fructose 1,6-bisphosphatase results in the substrate channeling. | Rakus, D., et al. 2004. Biochemistry. 43: 14948-57. PMID: 15554702
- Nuclear localization of fructose 1,6-bisphosphatase in smooth muscle cells. | Gizak, A., et al. 2005. J Mol Histol. 36: 243-8. PMID: 16200456
- Purification of human liver fructose-1,6-bisphosphatase. | Dzugaj, A. and Kochman, M. 1980. Biochim Biophys Acta. 614: 407-12. PMID: 6250626
- Function, structure and evolution of fructose-1,6-bisphosphatase. | Marcus, F., et al. 1987. Arch Biol Med Exp. 20: 371-8. PMID: 8816077
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FBPase Antibody (C-10) | sc-166145 | 200 µg/ml | $322.00 | |||
FBPase Antibody (C-10): m-IgGκ BP-HRP Bundle | sc-521521 | 200 µg Ab, 40 µg BP | $361.00 | |||
FBPase Antibody (C-10): m-IgG2b BP-HRP Bundle | sc-548919 | 200 µg Ab; 10 µg BP | $361.00 | |||
FBPase Antibody (C-10) AC | sc-166145 AC | 500 µg/ml, 25% agarose | $424.00 | |||
FBPase Antibody (C-10) HRP | sc-166145 HRP | 200 µg/ml | $322.00 | |||
FBPase Antibody (C-10) FITC | sc-166145 FITC | 200 µg/ml | $336.00 | |||
FBPase Antibody (C-10) PE | sc-166145 PE | 200 µg/ml | $349.00 | |||
FBPase Antibody (C-10) Alexa Fluor® 488 | sc-166145 AF488 | 200 µg/ml | $364.00 | |||
FBPase Antibody (C-10) Alexa Fluor® 546 | sc-166145 AF546 | 200 µg/ml | $364.00 | |||
FBPase Antibody (C-10) Alexa Fluor® 594 | sc-166145 AF594 | 200 µg/ml | $364.00 | |||
FBPase Antibody (C-10) Alexa Fluor® 647 | sc-166145 AF647 | 200 µg/ml | $364.00 | |||
FBPase Antibody (C-10) Alexa Fluor® 680 | sc-166145 AF680 | 200 µg/ml | $364.00 | |||
FBPase Antibody (C-10) Alexa Fluor® 790 | sc-166145 AF790 | 200 µg/ml | $364.00 |