ephrin-A3 Double Nickase Plasmid (h): sc-402771-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ephrin-A3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ephrin-A3 Double Nickase Plasmid (h) and ephrin-A3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EFNA3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ephrin-A3 Antibody (D-8): sc-393727
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ephrin-A3 Double Nickase Plasmid (h) | sc-402771-NIC | 20 µg | $410.00 | |||
ephrin-A3 Double Nickase Plasmid (h2) | sc-402771-NIC-2 | 20 µg | $410.00 |
EFNA3 encodes ephrin-A3, a glycosylphosphatidylinositol-anchored ligand for Eph receptor tyrosine kinases that mediates contact-dependent signaling to coordinate cell positioning, boundary formation, and axon guidance. Ephrin-A3–Eph interactions regulate cytoskeletal remodeling, adhesion dynamics, and bidirectional signaling that influences migration and tissue patterning through pathways linked to Rho family GTPases and MAPK/ERK signaling. In human biology, altered ephrin/Eph signaling has been associated with dysregulated angiogenesis, invasive behavior, and microenvironmental crosstalk in multiple disease contexts, including cancer. EFNA3 is therefore studied in mechanisms of cell–cell communication, developmental programs, and remodeling processes relevant to tumor progression and vascular biology.
ephrin-A3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EFNA3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EFNA3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EFNA3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EFNA3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.