EP58 Antibody (C-3): sc-390065

EP58 Antibody (C-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects EP58 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EP58 (C-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EP58, a novel ligand ER protein, is primarily expressed in the embryo, placenta, and adult heart, and exhibits unique structural characteristics that distinguish EP58 from other known endoplasmic reticulum-resident proteins. Notably, EP58 shares sequence similarity with bacterial and fungal proteins, suggesting a potential role in polysaccharide biosynthesis, which is crucial for various cellular processes, including cell wall formation and signaling. Understanding EP58 structure and interactions may provide insights into EP58 function in these biosynthetic pathways, highlighting EP58 importance in both developmental biology and potential therapeutic applications. The retention of soluble proteins in the endoplasmic reticulum is facilitated by the KDEL sequence, which interacts with the KDEL receptor in the Golgi apparatus, ensuring proper protein localization and function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

EP58 Antibody (C-3) References:

1. Identification of a novel mammalian endoplasmic reticulum-resident KDEL protein using an EST database motif search.  |  Kimata, Y., et al. 2000. Gene. 261: 321-7. PMID: 11167020
2. ER-to-Golgi transport and cytoskeletal interactions in animal cells.  |  Murshid, A. and Presley, JF. 2004. Cell Mol Life Sci. 61: 133-45. PMID: 14745493
3. Signal-mediated sorting of membrane proteins between the endoplasmic reticulum and the golgi apparatus.  |  Teasdale, RD. and Jackson, MR. 1996. Annu Rev Cell Dev Biol. 12: 27-54. PMID: 8970721
4. The dynamic organisation of the secretory pathway.  |  Pelham, HR. 1996. Cell Struct Funct. 21: 413-9. PMID: 9118249
5. The KDEL receptor, ERD2, regulates intracellular traffic by recruiting a GTPase-activating protein for ARF1.  |  Aoe, T., et al. 1997. EMBO J. 16: 7305-16. PMID: 9405360
6. Identification of amino acids in the binding pocket of the human KDEL receptor.  |  Scheel, AA. and Pelham, HR. 1998. J Biol Chem. 273: 2467-72. PMID: 9442098
7. Modulation of intracellular transport by transported proteins: insight from regulation of COPI-mediated transport.  |  Aoe, T., et al. 1998. Proc Natl Acad Sci U S A. 95: 1624-9. PMID: 9465066

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EP58 Antibody (C-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-EP58 Antibody (C-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EP58 Antibody (C-3) Specifications

Alternate Product Name: EP58

Product Clone Name: C-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 21-78

Epitope Target: EP58

Epitope Species: human

Product Reactivity/Detection: EP58

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for EP58 Antibody (C-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin