ENL Antibody (D-6): sc-393196
- ENL Antibody (D-6) is a mouse monoclonal IgG1 κ ENL antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 131-188 mapping within an internal region of ENL of human origin
- ENL Antibody (D-6) is recommended for detection of ENL of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ENL Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ENL Antibody (D-6). This work is in progress.
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SEE ALSO...
ENL Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects ENL of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ENL (D-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ENL, also known as MLLT1, plays a crucial role in transcriptional regulation and is involved in the formation of MLL fusion proteins, which are significant in the context of leukemia. ENL interacts with several key partners, including the MLL gene, which influences myelomonocytic differentiation. Fusion of ENL with MLL, resulting from chromosomal translocations, leads to the production of MLL-ENL fusion protein, commonly associated with infant acute leukemias. This interaction alters normal gene expression patterns, contributing to the pathogenesis of various leukemias. ENL activation of transcription from synthetic reporter genes in both lymphoid and myeloid cells underscores ENL′s importance in hematopoietic malignancies, making anti-ENL antibody (D-6) an essential tool for researchers studying these complex diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ENL Antibody (D-6) References:
- Physical interaction and functional antagonism between the RNA polymerase II elongation factor ELL and p53. | Shinobu, N., et al. 1999. J Biol Chem. 274: 17003-10. PMID: 10358050
- DNA structural properties of AF9 are similar to MLL and could act as recombination hot spots resulting in MLL/AF9 translocations and leukemogenesis. | Strissel, PL., et al. 2000. Hum Mol Genet. 9: 1671-9. PMID: 10861294
- Loss-of-function mutation of the AF9/MLLT3 gene in a girl with neuromotor development delay, cerebellar ataxia, and epilepsy. | Pramparo, T., et al. 2005. Hum Genet. 118: 76-81. PMID: 16001262
- Local gene density predicts the spatial position of genetic loci in the interphase nucleus. | Murmann, AE., et al. 2005. Exp Cell Res. 311: 14-26. PMID: 16202404
- Continuous MLL-ENL expression is necessary to establish a 'Hox Code' and maintain immortalization of hematopoietic progenitor cells. | Horton, SJ., et al. 2005. Cancer Res. 65: 9245-52. PMID: 16230385
- The human ALL-1/MLL/HRX antigen is predominantly localized in the nucleus of resting and proliferating peripheral blood mononuclear cells. | Ennas, MG., et al. 1997. Cancer Res. 57: 2035-41. PMID: 9158002
- Factors regulating the transcriptional elongation activity of RNA polymerase II. | Shilatifard, A. 1998. FASEB J. 12: 1437-46. PMID: 9806752
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ENL Antibody (D-6) | sc-393196 | 200 µg/ml | $322.00 | |||
ENL Antibody (D-6) AC | sc-393196 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ENL Antibody (D-6) HRP | sc-393196 HRP | 200 µg/ml | $322.00 | |||
ENL Antibody (D-6) FITC | sc-393196 FITC | 200 µg/ml | $336.00 | |||
ENL Antibody (D-6) PE | sc-393196 PE | 200 µg/ml | $349.00 | |||
ENL Antibody (D-6) Alexa Fluor® 488 | sc-393196 AF488 | 200 µg/ml | $364.00 | |||
ENL Antibody (D-6) Alexa Fluor® 546 | sc-393196 AF546 | 200 µg/ml | $364.00 | |||
ENL Antibody (D-6) Alexa Fluor® 594 | sc-393196 AF594 | 200 µg/ml | $364.00 | |||
ENL Antibody (D-6) Alexa Fluor® 647 | sc-393196 AF647 | 200 µg/ml | $364.00 | |||
ENL Antibody (D-6) Alexa Fluor® 680 | sc-393196 AF680 | 200 µg/ml | $364.00 | |||
ENL Antibody (D-6) Alexa Fluor® 790 | sc-393196 AF790 | 200 µg/ml | $364.00 |