Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
eIF4B Double Nickase Plasmid (h): sc-402494-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eIF4B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- eIF4B Double Nickase Plasmid (h) and eIF4B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EIF4B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: eIF4B Antibody (D-4): sc-376062
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF4B Double Nickase Plasmid (h) | sc-402494-NIC | 20 µg | $410.00 | |||
eIF4B Double Nickase Plasmid (h2) | sc-402494-NIC-2 | 20 µg | $410.00 |
EIF4B encodes eIF4B, an accessory translation initiation factor that enhances RNA helicase activity of eIF4A and supports recruitment of ribosomes to structured mRNAs during cap-dependent initiation. Through its interactions with the eIF4F complex and regulatory phosphorylation downstream of PI3K–AKT–mTOR and MAPK signaling, eIF4B helps tune translational output during growth, stress responses, and cell-cycle progression. Altered EIF4B expression or activation state has been associated with dysregulated protein synthesis programs that contribute to oncogenic signaling and aberrant cellular proliferation. As a node linking signaling pathways to selective mRNA translation, EIF4B is frequently studied in mechanisms controlling proteostasis, stress granule dynamics, and translation-dependent regulation of apoptosis and metabolism.
eIF4B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EIF4B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EIF4B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EIF4B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EIF4B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.