eIF4AII Double Nickase Plasmid (h): sc-402758-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- eIF4AII Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- eIF4AII Double Nickase Plasmid (h) and eIF4AII Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting EIF4A2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: eIF4AII Antibody (H-5): sc-137148
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF4AII Double Nickase Plasmid (h) | sc-402758-NIC | 20 µg | $410.00 | |||
eIF4AII Double Nickase Plasmid (h2) | sc-402758-NIC-2 | 20 µg | $410.00 |
EIF4A2 encodes eIF4AII, a DEAD-box RNA helicase that functions within the eIF4F translation initiation complex to unwind structured 5′ UTRs and promote ribosome loading. By regulating cap-dependent translation, eIF4AII influences proteome output during growth, stress responses, and cell-cycle progression, and interfaces with RNA metabolism pathways that control mRNA stability and translational efficiency. Altered control of translation initiation is linked to dysregulated signaling and proteostasis, making EIF4A2 a useful node for studying oncogenic translation programs and RNA-dependent vulnerabilities. EIF4AII activity is also relevant to contexts where selective translation of structured transcripts shapes differentiation and stress-adaptive gene expression.
eIF4AII Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the EIF4A2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within EIF4A2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt EIF4A2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of EIF4A2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.