eIF2β Antibody (P-3): sc-9978
- eIF2β Antibody (P-3) is a mouse monoclonal IgG1 κ eIF2β antibody, cited in 6 publications, provided at 200 µg/ml
- raised against full length eIF2β of rat origin
- eIF2 beta Antibody (P-3) is recommended for detection of eIF2β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-eIF2 beta Antibody (P-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for eIF2β Antibody (P-3) for WB and IHC(P) applications. These reagents are now offered in bundles with eIF2β Antibody (P-3) (see ordering information below).
eIF2β Antibody (P-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects eIF2β of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-eIF2β antibody (P-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. eIF2β plays a crucial role in the regulation of protein synthesis initiation in eukaryotic cells, functioning as a key component of the eukaryotic initiation factor 2 (eIF2) complex. This complex is essential for the recruitment of the ribosomal subunit to the mRNA, facilitating the translation process. The eIF2 complex is composed of three subunits: eIF2α, eIF2β, and eIF2γ, with eIF2β being vital for the stability and function of the entire complex. Notably, the phosphorylation of eIF2α, which occurs in response to various stress signals, leads to a decrease in the overall protein synthesis, allowing the cell to conserve resources and manage stress effectively. Understanding the dynamics of eIF2β and its interactions within the eIF2 complex is important for elucidating the mechanisms of translational control and its implications in various diseases, including cancer and neurodegenerative disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
eIF2β Antibody (P-3) References:
- The phosphorylation state of eucaryotic initiation factor 2 alters translational efficiency of specific mRNAs. | Kaufman, RJ., et al. 1989. Mol Cell Biol. 9: 946-58. PMID: 2657393
- Binding and release of eukaryotic initiation factor eIF-2 and GTP during protein synthesis initiation. | Trachsel, H. and Staehelin, T. 1978. Proc Natl Acad Sci U S A. 75: 204-8. PMID: 272635
- Cloning and sequencing of complementary DNAs encoding the alpha-subunit of translational initiation factor eIF-2. Characterization of the protein and its messenger RNA. | Ernst, H., et al. 1987. J Biol Chem. 262: 1206-12. PMID: 2948954
- Structure of the beta subunit of translational initiation factor eIF-2. | Pathak, VK., et al. 1988. Cell. 54: 633-9. PMID: 3044606
- The activity of eukaryotic initiation factor eIF-2 in ternary complex formation with GTP and Met-tRNA. | Benne, R., et al. 1979. J Biol Chem. 254: 3201-5. PMID: 429345
- Translation initiation factor eIF-2. Cloning and expression of the human cDNA encoding the gamma-subunit. | Gaspar, NJ., et al. 1994. J Biol Chem. 269: 3415-22. PMID: 8106381
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF2β Antibody (P-3) | sc-9978 | 200 µg/ml | $322.00 | |||
eIF2β Antibody (P-3): m-IgG Fc BP-HRP Bundle | sc-528245 | 200 µg Ab; 10 µg BP | $361.00 | |||
eIF2β Antibody (P-3): m-IgGκ BP-HRP Bundle | sc-520572 | 200 µg Ab, 40 µg BP | $361.00 | |||
eIF2β Antibody (P-3): m-IgG1 BP-HRP Bundle | sc-542835 | 200 µg Ab; 20 µg BP | $361.00 | |||
eIF2β Antibody (P-3) AC | sc-9978 AC | 500 µg/ml, 25% agarose | $424.00 | |||
eIF2β Antibody (P-3) HRP | sc-9978 HRP | 200 µg/ml | $322.00 | |||
eIF2β Antibody (P-3) FITC | sc-9978 FITC | 200 µg/ml | $336.00 | |||
eIF2β Antibody (P-3) PE | sc-9978 PE | 200 µg/ml | $349.00 | |||
eIF2β Antibody (P-3) Alexa Fluor® 488 | sc-9978 AF488 | 200 µg/ml | $364.00 | |||
eIF2β Antibody (P-3) Alexa Fluor® 546 | sc-9978 AF546 | 200 µg/ml | $364.00 | |||
eIF2β Antibody (P-3) Alexa Fluor® 594 | sc-9978 AF594 | 200 µg/ml | $364.00 | |||
eIF2β Antibody (P-3) Alexa Fluor® 647 | sc-9978 AF647 | 200 µg/ml | $364.00 | |||
eIF2β Antibody (P-3) Alexa Fluor® 680 | sc-9978 AF680 | 200 µg/ml | $364.00 | |||
eIF2β Antibody (P-3) Alexa Fluor® 790 | sc-9978 AF790 | 200 µg/ml | $364.00 |