Dok-3 Antibody (H-5): sc-373885

Dok-3 Antibody (H-5) is a mouse monoclonal IgG1 antibody that detects Dok-3 in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Dok-3 is a crucial adapter protein that plays a significant role in recruiting inhibitory molecules, particularly in immune cells like B cells and macrophages. Expression of Dok-3 is notably elevated in these cell types, where Dok-3 becomes activated upon immunoreceptor-mediated cellular stimulation, leading to tyrosine phosphorylation. This post-translational modification enables Dok-3 to interact with key signaling proteins, including the 5′ inositol phosphatase SHIP and the protein tyrosine kinase Csk. Through binding to these proteins, Dok-3 is involved in the negative regulation of immunoreceptor signaling, thereby modulating immune responses and maintaining cellular homeostasis. The role of Dok-3 as a signaling hub highlights its importance in the intricate network of immune regulation, making anti-Dok-3 antibody (H-5) a valuable tool for researchers studying immune signaling pathways and their implications in health and disease.

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Dok-3 Antibody (H-5) References:

1. Inhibition of the Jun N-terminal protein kinase pathway by SHIP-1, a lipid phosphatase that interacts with the adaptor molecule Dok-3.  |  Robson, JD., et al. 2004. Mol Cell Biol. 24: 2332-43. PMID: 14993273
2. Subcellular localization of Grb2 by the adaptor protein Dok-3 restricts the intensity of Ca2+ signaling in B cells.  |  Stork, B., et al. 2007. EMBO J. 26: 1140-9. PMID: 17290227
3. Mice lacking Dok-1, Dok-2, and Dok-3 succumb to aggressive histiocytic sarcoma.  |  Mashima, R., et al. 2010. Lab Invest. 90: 1357-64. PMID: 20548287
4. B cell receptor-mediated antigen gathering requires ubiquitin ligase Cbl and adaptors Grb2 and Dok-3 to recruit dynein to the signaling microcluster.  |  Schnyder, T., et al. 2011. Immunity. 34: 905-18. PMID: 21703542
5. The Dok-3/Grb2 protein signal module attenuates Lyn kinase-dependent activation of Syk kinase in B cell antigen receptor microclusters.  |  Lösing, M., et al. 2013. J Biol Chem. 288: 2303-13. PMID: 23223229
6. The Dok-3/Grb2 adaptor module promotes inducible association of the lipid phosphatase SHIP with the BCR in a coreceptor-independent manner.  |  Manno, B., et al. 2016. Eur J Immunol. 46: 2520-2530. PMID: 27550373
7. What goes up must come down: A tripartite Dok-3/Grb2/SHIP1 inhibitory module limits BCR signaling.  |  Reth, M. and Gold, MR. 2016. Eur J Immunol. 46: 2507-2511. PMID: 27813071
8. Dok-3 and Dok-1/-2 adaptors play distinctive roles in cell fusion and proliferation during osteoclastogenesis and cooperatively protect mice from osteopenia.  |  Kajikawa, S., et al. 2018. Biochem Biophys Res Commun. 498: 967-974. PMID: 29548825
9. Dok-3 deficient mice display different immune clustering and Tim-3 expression.  |  Yan, W., et al. 2019. Eur J Med Res. 24: 26. PMID: 31351483
10. Cross-disease transcriptomic analysis reveals DOK3 and PAPOLA as therapeutic targets for neuroinflammatory and tumorigenic processes.  |  Wang, X., et al. 2024. Front Immunol. 15: 1504629. PMID: 39726593

The preferred Dok-3 antibody is Dok-3 Antibody (F-7): sc-390007.