Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cytokeratin 8 Antibody (C51) Alexa Fluor® 594: sc-8020 AF594. Direct fluorescent western blot analysis of Cytokeratin 8 expression in JAR (A), MCF7 (B), SK-BR-3 (C), BT-20 (D), PC-3 (E) and Hep G2 (F) whole cell lysates. Blocked with UltraCruz® Blocking Reagent: sc-516214.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cytokeratin 8 Double Nickase Plasmid (h): sc-418254-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cytokeratin 8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Cytokeratin 8 Double Nickase Plasmid (h) and Cytokeratin 8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KRT8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cytokeratin 8 Antibody (C51): sc-8020
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cytokeratin 8 Double Nickase Plasmid (h) | sc-418254-NIC | 20 µg | $410.00 | |||
Cytokeratin 8 Double Nickase Plasmid (h2) | sc-418254-NIC-2 | 20 µg | $410.00 |