SAM Transcription Activation: SAM system utilizing dCas9-VP64, MS2-P65-HSF1, and sgRNA for precise gene transcription activation.
Cytokeratin 12 CRISPR Activation Plasmid (h): sc-400955-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cytokeratin 12 CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- Cytokeratin 12 CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by Cytokeratin 12 CRISPR Activation Plasmid (h) and Cytokeratin 12 CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the KRT12 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cytokeratin 12 Antibody (E-8): sc-515882
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cytokeratin 12 CRISPR Activation Plasmid (h) | sc-400955-ACT | 20 µg | $397.00 |