CYPOR Antibody (F-2): sc-55477

CYPOR Antibody (F-2) is a mouse monoclonal IgG2a antibody that detects CYPOR in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CYPOR, also known as cytochrome P450 reductase, plays a crucial role in the metabolism of various endogenous and xenobiotic compounds by facilitating the transfer of electrons from NADPH to cytochrome P450 enzymes during their catalytic cycle. This electron transfer is essential for the proper functioning of the P450 enzymes, which are involved in the biotransformation of drugs and toxins, thereby influencing drug efficacy and toxicity. Located in the endoplasmic reticulum, CYPOR not only supports cytochrome P450 enzymes but also transfers electrons to heme oxygenase and cytochrome b5, highlighting CYPOR′s importance in multiple metabolic pathways. Structurally, CYPOR is related to two distinct flavoprotein families, ferredoxin nucleotide reductase and flavodoxin, and CYPOR′s electron transfer activity relies on the binding of two flavin cofactors, FAD and FMN, to respective domains. Anti-CYPOR antibody (F-2) serves as an invaluable tool for researchers studying drug metabolism and the biochemical pathways involving cytochrome P450 enzymes, providing insights into their regulatory mechanisms and interactions.

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CYPOR Antibody (F-2) References:

1. The cytochrome P450 2B4-NADPH cytochrome P450 reductase electron transfer complex is not formed by charge-pairing.  |  Voznesensky, AI. and Schenkman, JB. 1992. J Biol Chem. 267: 14669-76. PMID: 1321814
2. Measurement of cytochrome P450 and NADPH-cytochrome P450 reductase.  |  Guengerich, FP., et al. 2009. Nat Protoc. 4: 1245-51. PMID: 19661994
3. Cytochrome P450 2S1 is reduced by NADPH-cytochrome P450 reductase.  |  Xiao, Y., et al. 2011. Drug Metab Dispos. 39: 944-6. PMID: 21430234
4. Gating mechanisms for biological electron transfer: integrating structure with biophysics reveals the nature of redox control in cytochrome P450 reductase and copper-dependent nitrite reductase.  |  Leferink, NG., et al. 2012. FEBS Lett. 586: 578-84. PMID: 21762695
5. H-rev107 Regulates Cytochrome P450 Reductase Activity and Increases Lipid Accumulation.  |  Tsai, FM., et al. 2015. PLoS One. 10: e0138586. PMID: 26381418
6. NADPH-cytochrome P450 reductase-mediated denitration reaction of 2,4,6-trinitrotoluene to yield nitrite in mammals.  |  Shinkai, Y., et al. 2016. Free Radic Biol Med. 91: 178-87. PMID: 26454083
7. Protonation of the Hydroperoxo Intermediate of Cytochrome P450 2B4 Is Slower in the Presence of Cytochrome P450 Reductase Than in the Presence of Cytochrome b5.  |  Pearl, NM., et al. 2016. Biochemistry. 55: 6558-6567. PMID: 27797496
8. Effector role of cytochrome P450 reductase for androstenedione binding to human aromatase.  |  Zhang, C., et al. 2020. Int J Biol Macromol. 164: 510-517. PMID: 32698066
9. Structural Dynamics of Cytochrome P450 3A4 in the Presence of Substrates and Cytochrome P450 Reductase.  |  Ducharme, J., et al. 2021. Biochemistry. 60: 2259-2271. PMID: 34196520
10. Effects of Diminished NADPH:cytochrome P450 Reductase in Human Hepatocytes on Lipid and Bile Acid Homeostasis.  |  Heintze, T., et al. 2021. Front Pharmacol. 12: 769703. PMID: 34867397

The preferred CYPOR antibody is CYPOR Antibody (F-10): sc-25270.