cyclin D2 Anticuerpo (DCS-5) recomendado para detectarcyclin D2 de mouse, rat y human origen, mediante WB, IP, IF, FCM y ELISA

cyclin D2 Anticuerpo (DCS-5): sc-53637

cyclin D2 Anticuerpo (DCS-5) clasificado 4.5 de 5 por 6.
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Ficha técnica

    • cyclin D2 Anticuerpo (DCS-5) es un monoclonal de ratón IgG2b κ, ver las 12 publicaciones, proporcionado como 200 µg/ml
    • producido contra la proteína completa cyclin D2 de origen human
    • cyclin D2 Anticuerpo (DCS-5) es recomendado para detectar cyclin D2 de mouse, rat y human origen, mediante WB, IP, IF, FCM y ELISA
    • cyclin D2 Anticuerpo (DCS-5) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de cyclin D2 (DCS-5): sc-53637.

Can cyclin D2 (DCS-5): sc-53637 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Preguntado por: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, cyclin D2 (DCS-5): sc-53637 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Respondida por: Technical Support
Fecha de publicación: 2017-02-25
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Rated 5 de 5 por de Specific Western detection in rat INS-1 cells 60 ug loads of rat insulinoma (INS-1) lysates were probed overnight and returned a strong, specific band of ~38 kD. Antibody sc-56305 is also suitable. The attached picture is a triplicate set (identical lanes)
Fecha de publicación: 2019-05-22
Rated 5 de 5 por de Nice signal by WB on mouse colon extracts This antibody works perfectly on mouse colon extracts (20 ug) at a 1/200 dilution with regular ECL.
Fecha de publicación: 2017-10-27
Rated 4 de 5 por de a relative good antibody for WB This antibody generates a signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract). However, I would recommend to use sc-376676 for WB.
Fecha de publicación: 2017-09-18
Rated 3 de 5 por de not very clean In HeLa, it is a low-affinity antibody. Not very clean, it has many nonspecific larger MW bands which are stronger than the cyclin-D2 band.
Fecha de publicación: 2017-04-12
Rated 5 de 5 por de Mono works in human transfected lysates Mono works in human transfected lysates. -SCBT QC
Fecha de publicación: 2015-02-12
Rated 5 de 5 por de Works well in MM Works well in MM-142 nuclear extract under reducing and non-reducing conditions. -SCBT QC
Fecha de publicación: 2014-07-17
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