Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CTGF Double Nickase Plasmid (h): sc-400091-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CTGF Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CTGF Double Nickase Plasmid (h) and CTGF Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CTGF. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CTGF Antibody (E-5): sc-365970
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CTGF Double Nickase Plasmid (h) | sc-400091-NIC | 20 µg | $410.00 | |||
CTGF Double Nickase Plasmid (h2) | sc-400091-NIC-2 | 20 µg | $410.00 |
Connective tissue growth factor (CTGF/CCN2) is a secreted matricellular protein that modulates cell–matrix interactions, proliferation, migration, and extracellular matrix (ECM) deposition. It functions downstream of TGF-β/SMAD signaling and integrates cues from integrins, MAPK, and YAP/TAZ mechanotransduction pathways to coordinate fibroblast activation and tissue remodeling. Dysregulated CTGF expression is linked to fibrotic programs and aberrant stromal remodeling, with broad relevance to organ fibrosis biology and tumor microenvironment research. In vitro, CTGF is commonly studied for its roles in myofibroblast differentiation, collagen synthesis, angiogenic responses, and regulation of inflammatory cell crosstalk within remodeling tissues.
CTGF Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CTGF locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CTGF. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CTGF function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CTGF-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.