Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CRX CRISPR/Cas9 KO Plasmid (m): sc-419817
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CRX CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CRX genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CRX Antibody (A-9): sc-377138
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CRX CRISPR/Cas9 KO Plasmid (m) | sc-419817 | 20 µg | $397.00 |
Overview
Crx encodes the cone-rod homeobox (CRX) transcription factor, a master regulator of photoreceptor differentiation and maintenance in the mouse retina. CRX binds cis-regulatory elements to drive expression of genes required for phototransduction, outer segment biogenesis, and synaptic function, integrating transcriptional networks with other retinal lineage factors. Disruption of CRX-dependent gene programs impairs maturation and survival of rods and cones, linking this pathway to inherited retinal degeneration phenotypes. Accordingly, Crx is widely studied for mechanisms of retinal development, photoreceptor gene regulation, and genotype–phenotype relationships in visual system disease models.
CRX CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Crx gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Crx together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Crx open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CRX protein expression.
This CRISPR knockout system enables efficient generation of Crx-deficient cell models for investigation of CRX signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Crx exon(s) critical for CRX function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Crx genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CRX CRISPR/Cas9 KO Plasmid (m) and CRX CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Crx locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CRX HDR Plasmid (m) and CRX HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Crx homology arms to support homology-directed repair at defined Crx target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.