Crossveinless-2 CRISPR/Cas9 KO Plasmid (h): sc-406614
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Crossveinless-2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Crossveinless-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Crossveinless-2 Antibody (G-8): sc-377502
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Crossveinless-2 CRISPR/Cas9 KO Plasmid (h) | sc-406614 | 20 µg | $397.00 |
Overview
BMPER encodes the secreted extracellular matrix glycoprotein Crossveinless-2, a context-dependent regulator of bone morphogenetic protein (BMP) signaling. By binding BMP ligands and modulating their availability to BMP receptors, BMPER influences SMAD-dependent transcriptional programs that control endothelial behavior, tissue morphogenesis, and extracellular matrix remodeling. BMPER activity intersects with developmental and vascular homeostasis pathways and has been linked in the literature to dysregulated angiogenic signaling and connective tissue phenotypes when perturbed. These properties make BMPER a useful target for studying BMP gradient formation, ligand sequestration, and pathway tuning in cell and organoid models.
Crossveinless-2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BMPER gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BMPER together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BMPER open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Crossveinless-2 protein expression.
This CRISPR knockout system enables efficient generation of BMPER-deficient cell models for investigation of Crossveinless-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BMPER exon(s) critical for Crossveinless-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BMPER genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Crossveinless-2 CRISPR/Cas9 KO Plasmid (h) and Crossveinless-2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BMPER locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Crossveinless-2 HDR Plasmid (h) and Crossveinless-2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BMPER homology arms to support homology-directed repair at defined BMPER target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.